CD278 [ICOS] (human):Fc (human) (recombinant protein) (non-lytic)

Specifications / Handling

More Information
Product Details
Synonyms	ICOS; Inducible T Cell Costimulator; CRP1; CD28-related Protein-1; AILIM
Product Type	Protein
Properties
Source/Host	CHO cells
Sequence	The extracellular domain of human CD278 [ICOS] (aa 21-139) is fused to the N-terminus of the Fc region of a mutant human IgG1.
Crossreactivity	Human
Biological Activity	Shows the biological function of the CD278 moiety and exerts a prolonged circulating half-life caused by the modified Fc domain.
Purity	≥98% (SDS-PAGE)
Endotoxin Content	<0.06EU/μg protein (LAL test; Lonza).
Reconstitution	Reconstitute at 100μg/ml in sterile PBS.
Formulation	Lyophilized from 0.2μm-filtered solution in PBS.
Protein Negative Control	Fc (human) IgG1 Control (rec.) (non-lytic)
Other Product Data	Non-lytic: Acts as a long lasting fusion protein which only binds to the receptor. Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC). NCBI reference NP_036224.1: CD278 [ICOS] (human)
Declaration	Manufactured by Chimerigen.
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS	Inquire
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

CD278, also called inducible costimulator (ICOS) or CRP1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. ICOS is expressed on most CD45RO⁺ cells. ICOS expression is upregulated within approximately 24-48 hours of activation on Th primed cells. B7-H2, a member of the B7 family of costimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.

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CD278 [ICOS] (human):Fc (human) (rec.) (non-lytic)