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eTOPIX_Stabilizing Biological Macromolecules with Extremolytes – Sent on 16 March 2021
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| eTOPIX Product Highlights - 16 March 2021 | www.adipogen.com | ||||||||||
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 Extremolytes & BioStab Ready-to-Use Stabilizers of Biological Structures and Macromolecules |
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AdipoGen Life Sciences, in collaboration with bitop AG, offers a range of potent extremolytes. This includes the pure solid compounds Ectoine, Hydroxyectoine, Glucosylglycerol and the Ready-to-Use BioStab solutions for biomolecule stabilization in life science and diagnostics applications. The Ready-to-Use BioStab buffers are mixtures containing the before mentioned stabilizing compounds. These BioStab solutions are colourless, animal-free, protein-free and serum-free solutions and compatible with enzymatic or binding reactions. They provide increased stability of biological structures (e.g. proteins, enzymes, DNA, RNA), protection during freeze-drying, freeze-thaw cycles and heat, long term storage of antibodies without loss of activity and preservation of binding activity of antibodies.  AdipoGen Life Sciences' BioStab Peroxidase Conjugate Stabilizer (Prod. No. AG-10T-0030) extends the lifetime of HRP-antibody conjugates and increases the signal intensity, allowing much more consistent results over extended periods of time compared to daily prepared dilutions. The mode of action of BioStab HRP Stabilizer comprises both stabilization of the antibody and stabilization of the enzymatically active centre of HRP. The BioStab Peroxidase Conjugate Stabilizer can be used in ELISA, Western Blotting, Immunohistochemistry and any other applications which require HRP conjugates. Figure: BioStab Peroxidase Conjugate Stabilizer can be used in immunoassays, such as common sandwich ELISA systems, for long term storage of diluted HRP-conjugated antibodies without loss of activity. Real-time studies showed that after storage for 24 months at RT or 4°C, residual activity comprises more than 60%. The conjugate was diluted 1:10'000 in BioStab Peroxidase Conjugate Stabilizer.   AdipoGen Life Sciences' BioStab Antibody Stabilizer (Prod. No. AG-10T-0031) dramatically extends the life of your valuable antibodies, allowing much more consistent results over extended periods of time compared to daily prepared dilutions. The mode of action of BioStab Antibody Stabilizer comprises direct stabilization of the antibody by minimization of unspecific interactions. The BioStab Antibody Stabilizer can be used in ELISA, Western Blotting, Immunohistochemistry and any other applications which require free or biotinylated antibodies. Figure: Antibodies diluted in BioStab Antibody Stabilizer can be frozen without loss of binding activity even after three freeze/thaw cycles. Stabilization capacity of BioStab Antibody Stabilizer in comparison with a competitor's product. Remaining activity of an antibody (Rabbit Ig diluted 5ng/ml) after three freeze/thaw cycles (-80°C/+65°C).  PCR is a technique extensively used in different applications in molecular biology, molecular diagnostics and forensics. However, some sequences, for example the 5´-regions of many eukaryotic genes, are difficult to amplify. Incomplete separation of DNA strands results in little or no yield of desired product and multiple non-specific PCR-fragments. AdipoGen Life Sciences' BioStab PCR Optimizer (Prod. No. AG-10T-0032) solves this problem and outperforms betaine. The mode of action of BioStab PCR Optimizer comprises lowering the melting temperature of GC pairs without affecting that of AT pairs. The BioStab PCR Optimizer is designed as a PCR additive which improves the yield and specificity of PCR reaction and allows amplification of difficult targets (e.g. sequences with >70% GC pairs). The BioStab PCR Optimizer is provided as 5x concentrated solution. Figure: Amplification of a 375 bp fragment from the 5'-region of mouse cAMP-dependent protein kinase Cβ gene (74% GC) using different annealing temperature: BioStab PCR Optimizer allows the use of a wide range of annealing temperatures thereby eliminating the need for an optimization of amplification conditions. Lanes 2-4 show the PCR product obtained in the presence of BioStab PCR Optimizer at different annealing temperatures, lanes 5-7 show the result of a PCR-reaction without BioStab PCR Optimizer. Lane 1 - DNA Ladder. High Purity Extremolytes!
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