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AdipoGen Life Sciences
Fc (human):Grancalcin (human) (rec.)
440
CHF
CHF 440.00
In stock
AG-40B-0242-C05050 µgCHF 440.00
| Product Details | |
|---|---|
| Synonyms | GCA; GCL |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells |
| Sequence |
Human Grancalcin (aa 1-217) is fused at the N-terminus to the Fc portion of human IgG1. |
| Crossreactivity | Human |
| MW | ~65kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.01EU/μg purified protein (LAL test). |
| Concentration | 1mg/ml after reconstitution. |
| Reconstitution | Reconstitute with 50µl endotoxin-free water. |
| Accession Number | P28676 |
| Formulation | Lyophilized. Contains PBS. |
| Protein Negative Control | |
| Other Product Data |
Uniprot link P28676: Grancalcin (human) |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
Grancalcin is a member of a new family of proteins named penta-EF-hand (PEF), which contains five repetitive EF hand motifs. PEF proteins form a unique group including calpain, sorcin, grancalcin, ALG-2 (apoptosis-linked gene-2 protein) and peflin. The penta-EF hand members are Ca2+-binding proteins implicated in regulating cell migration, apoptosis and the mobilization of immune cells.
Senescent cells accumulate in the bone marrow and secrete factors, termed senescence-associated secretory phenotype (SASP), that can promote skeletal aging. Recently, senescent neutrophils and macrophages in the bone marrow were shown to be critical cell types during skeletal aging releasing Grancalcin (GCA) to promote such aging. Grancalcin seems to act by competitively binding to and inactivating PlexinB2 functions in bone marrow stromal cells (BMSCs) leading to repression of osteogenesis and increase of adipogenesis. Increased Grancalcin expression in immune cells (bone marrow macrophages and in neutrophils) is due to higher endotoxin levels observed during aging.