UIBC Colorimetric Assay Kit (Bathophenanthroline Method)

CHF 553.00
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JAI-CBC-800100 testsCHF 553.00
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Product Details
Synonyms Unsaturated Iron Binding Capacity Assay Kit
Product Type Kit
Application Set Quantitative ELISA
Specificity Detection of unsaturated iron binding capacity (UIBC) by measuring a proportional difference between Fe2+ and Fe3+.
Crossreactivity All
Quantity Enough reagents for 100 tests.
Range 10 - 800μg/dL
Sample Type Plasma
Detection Type Colorimetric
Kit Contains 1 x 20 ml  R-1   Buffer (Iron concentration 80μg/dL) (ready to use)
1 x   3 ml  R-2   Chelate color (ready to use)
Other Product Data This MC-Reagent UIBC Assay Kit is intended for the quantitative determination of unsaturated iron binding capacity (UIBC) in samples by 96-well reader use. The kit utilizes the chromogen bathophenanthroline to bind Fe2+. Fe3+ is then reduced to Fe2+ by a reducing agent. The Fe2+ reacts with bathophenanthroline to form a colored complex which is measured by 96-well reader or spectrophotometer and is proportional to the amount of iron present. Absorbance of the Fe2+-complex is measured at 546nm and 600nm (reference wavelength).

EDTA-Plasma samples cannot be used due to interference with the chromogenic system.
Declaration Manufactured by JaICA.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage +4°C
Handling Advice Do not freeze.
Use/Stability 12 months after the day of manufacturing. See expiry date on ELISA Kit box.
Manual Download PDF
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Iron is an essential element in mammalians and is contained in various enzymes and is involved in oxidation reactions. Iron is essential during transport of oxygen as composition element of hemoglobin or myoglobin. 30% of the transferrin is associated with Fe3+ in blood. Free transferrin is not associated to iron. TIBC (Total iron binding capacity) = UIBC (Unsaturated iron binding capacity) + Serum iron. TIBC levels change in blood disorders, hepatic diseases, tumors and inflammation. UIBC levels are increased in patients with iron deficiency. Decreased levels of UIBC are seen in patients with infectious diseases, nephrotic syndrome and low proteinosis.

Product References
  1. Biliary excretion of excess iron in mice requires hepatocyte iron import by Slc39a14: M. Prajapati, et al.; J. Biol. Chem. 297, 100835 (2021) 
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