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RevMab
anti-Phosphothreonine, Rabbit Monoclonal (RM102)
518
CHF
CHF 518.00
In stock
REV-31-1009-00-C100100 µgCHF 518.00
Figure 1: Immunocytochemistry of serum-starved A431 cells nontreated or treated with Calyculin A/ Okadaic Acid
| Product Details | |
|---|---|
| Synonyms | pThr |
| Product Type | Recombinant Antibody |
| Properties | |
| Clone | RM102 |
| Isotype | Rabbit IgG |
| Source/Host | Rabbit |
| Immunogen/Antigen | Mixture of phosphothreonine-BSA conjugate and a phosphothreonine containing peptide. |
| Application |
Western Blot (WB): 1:500 -1:2000 dilution |
| Crossreactivity | All |
| Specificity |
This antibody reacts to threonine-phosphorylated proteins. No cross reactivity with nonphosphorylated threonine, phosphoserine, and phosphotyrosine. It shows slight cross-reactivity with a few phospho-serine-containing peptides. |
| Purity | Protein A purified. |
| Purity Detail | Protein A affinity purified from an animal origin-free culture supernatant. |
| Concentration | 1 mg/ml |
| Formulation | Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. |
| Isotype Negative Control | |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| Accession Number | N/A |
| Declaration | Manufactured by RevMab Biosciences. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
Threonine is an essential amino acid that is used in the biosynthesis of proteins. The threonine residue is susceptible to numerous posttranslational modifications (PTMs). The hydroxyl side-chain can undergo O-linked glycosylation. In addition, threonine residues undergo phosphorylation through the action of a threonine kinase. In its phosphorylated form, it can be referred to as phosphothreonine. Phosphothreonine has three potential coordination sites (carboxyl, amine and phosphate group) and determination of the mode of coordination between phosphorylated ligands and metal ions occurring in an organism is important to explain the function of the phosphothreonine in biological processes.