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RevMab
anti-GAPDH, Rabbit Monoclonal (RM114) (Biotin)
459
CHF
CHF 459.00
In stock
REV-31-1017-02-R05050 µlCHF 459.00
Figure 1: Immunocytochemical staining of HeLa cells, using anti-GAPDH RevMAb Clone RM114 at 1/200 dilution (red). Nuclei have been labeled with DAPI (blue).
| Product Details | |
|---|---|
| Synonyms | Glyceraldehyde-3-phosphate Dehydrogenase; Peptidyl-cysteine S-nitrosylase GAPDH |
| Product Type | Recombinant Antibody |
| Properties | |
| Clone | RM114 |
| Isotype | Rabbit IgG |
| Source/Host | Rabbit |
| Immunogen/Antigen | A peptide corresponding to the C-terminus of GAPDH. |
| Label/Conjugates | Biotin |
| Application |
Western Blot (WB): 1:1000 dilution Immunoprecipitation (IP): 1:200 dilution Chromatin IP (ChIP): 1:200 dilution Immunocytochemistry (ICC): 1:200 dilution Immunohistochemistry (IHC): 1:200 dilution |
| Crossreactivity |
Goat Human Monkey Mouse |
| Specificity | This antibody reacts to GAPDH. RM114 shows strong reactivity to Human/Monkey GAPDH. RM114 shows weaker reactivity to Mouse, Goat GAPDH and may require higher antibody dilution usage. |
| Purity | Protein A purified. |
| Purity Detail | Protein A affinity purified from an animal origin-free culture supernatant. |
| Concentration | N/A |
| Formulation | Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| Accession Number | P04406 |
| Declaration | Manufactured by RevMab Biosciences. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) is an enzyme of ~37kDa which is a tetramer composed of four identical 37-kDa subunits, containing a single catalytic thiol group each and critical to the enzyme's catalytic function. GADPH catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, ER to Golgi vesicle shuttling, DNA repair. membrane fusion, microtubule bundling and age-related actions in neurodegenerative diseases.