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RevMab
IgG3 (human) Matched Antibody Pair
629
CHF
CHF 629.00
In stock
REV-31-1021-MK-KI011 SetCHF 629.00
Figure 1: Sandwich ELISA, using RevMAb human IgG3 matched antibody pair, shows species reactivity to human only, and shows no cross-reactivity to monkey (Cyno or Rhesus), mouse IgG, rat IgG, or goat IgG.
| Product Details | |
|---|---|
| Synonyms | Immunoglobulin G3 |
| Product Type | Set |
| Properties | |
| Application Set | Quantitative ELISA |
| Specificity | The antibody pair detects only human IgG3. It does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG. There is no cross reactivity to any other immunoglobulins or IgG subclasses. |
| Crossreactivity | Human |
| Quantity |
ELISA: Capture Antibody 50ng/well - 200ng/well Detection Antibody 0.1ug/mL - 0.5ug/mL |
| Sample Type | Serum |
| Assay Type | Sandwich |
| Kit Contains |
1) Capture Antibody: Anti-Human IgG3 Rabbit Monoclonal antibody, clone RM119, 100 ug at 1.0 mg/ml in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide 2) Detection Antibody: Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody, Clone RM129, 25 ug at 1.0 mg/ml in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide 3) 10X Sample Diluent: Added to the tested sample to reduce non-specific background and noise |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| Declaration | Manufactured by RevMab Biosciences. |
| Accession Number | P01860 |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| Manual | No |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
The Human IgG3 Matched Antibody Pairs is specific to Human IgG3 and has been demonstrated through ELISA to not recognize (cross) IgG from Non-Human Primate species (Rhesus and Cynomologus macaques), nor does the matched pair recognize mouse, rat or goat immunoglobulin. The kit can be used for the detection of therapeutic antibodies from samples obtained from experimental animals to provide accurate pharmacokinetic (PK) data.
IgG (Immunoglobulin G) is the most abundant Ig isotype in serum and makes up approximately 75% of all serum Immunoglobulins. In humans, there are four subclasses of IgG with highest serum concentrations of IgG1 followed by IgG2, IgG3 and IgG4. The IgG molecule consists of two heavy and two lights chains (κ or λ) resulting in a molecule with two arms for antigen binding. High levels of IgG antibodies are induced following the initial IgM response in a typical immune response to antigens. Because of its relative abundance and excellent specificity toward antigens, IgG is the principle antibody used in immunological research and clinical diagnostics. Clinically, measured IgG antibody levels are generally considered to be indicative of an individual's immune status to particular pathogens.