RevMab

anti-His-Tag, Rabbit Monoclonal (RM146)

CHF 489.00
In stock
REV-31-1048-00-C100100 µgCHF 489.00
More Information
Product Details
Synonyms Polyhistidin
Product Type Recombinant Antibody
Properties
Clone RM146
Isotype Rabbit IgG
Source/Host Rabbit
Immunogen/Antigen Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.
Application

Western Blot: 0.1 µg/ml-1 µg/ml
Immunoprecipitation: 0.5 µg/ml-2 µg/ml
Immunocytochemistry 0.5 µg/ml-2 µg/ml
Flow Cytometry: 0.5 µg/ml-2 µg/ml
Immunohistochemistry
0.1 µg/ml-1 µg/ml

Crossreactivity All
Specificity

This antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.

Purity Protein A purified.
Purity Detail Protein A affinity purified from an animal origin-free culture supernatant.
Concentration 1 mg/ml
Formulation Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide.
Isotype Negative Control

Rabbit IgG

Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

Accession Number N/A
Declaration Manufactured by RevMab Biosciences.
Shipping and Handling
Shipping BLUE ICE
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF
Description

His-tag is a series of six to nine histidine residues fused to either the carboxyl or amino terminus of a recombinant protein. The small size of the His-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions allows for the simple purification and detection of His-tagged proteins. His-tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe. This His-tag antibody is also available biotin-labeled to meet all your research needs.

© 2017 Adipogen Life Sciences. Pictures: © 2012 Martin Oeggerli. All Rights Reserved.