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RevMab
anti-His-Tag, Rabbit Monoclonal (RM146) (Biotin)
489
CHF
CHF 489.00
In stock
REV-31-1048-02-C05050 µgCHF 489.00
Figure 1: Immunohistochemistry staining of naive HepG2 cells (Negative control) using anti-His-Tag antibody, RM146.
| Product Details | |
|---|---|
| Synonyms | Polyhistidin |
| Product Type | Recombinant Antibody |
| Properties | |
| Clone | RM146 |
| Isotype | Rabbit IgG |
| Source/Host | Rabbit |
| Immunogen/Antigen | Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus. |
| Label/Conjugates | Biotin |
| Application |
Western Blot: 0.1 µg/ml-0.5 µg/ml Immunocytochemistry 0.5 µg/ml-2 µg/ml Flow Cytometry: 0.5 µg/ml-2 µg/ml Immunohistochemistry 0.1 µg/ml-1 µg/ml ELISA: 0.01 µg/ml-0.5 µg/ml |
| Crossreactivity | All |
| Specificity | This antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells. |
| Purity | Protein A purified. |
| Purity Detail | Protein A affinity purified from an animal origin-free culture supernatant. |
| Concentration | 1 mg/ml |
| Formulation | Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| Accession Number | N/A |
| Declaration | Manufactured by RevMab Biosciences. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
His-tag is a series of six to nine histidine residues fused to either the carboxyl or amino terminus of a recombinant protein. The small size of the His-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions allows for the simple purification and detection of His-tagged proteins. His-tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe. This His-tag antibody is also available biotin-labeled to meet all your research needs.