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RevMab
anti-Gamma H2AX (phospho-Ser139), Rabbit Monoclonal (RM224)
251
CHF
CHF 251.00
In stock
REV-31-1104-00-C02525 µgCHF 251.00
REV-31-1104-00-C100100 µgCHF 559.00
Figure 1: Immunocytochemistry of HeLa cells using gamma H2AX Rabbit mAb RM224 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
| Product Details | |
|---|---|
| Synonyms | H2AX pSer139 |
| Product Type | Recombinant Antibody |
| Properties | |
| Clone | RM224 |
| Isotype | Rabbit IgG |
| Source/Host | Rabbit |
| Immunogen/Antigen | A phospho-peptide corresponding to Phospho-Histone H2AX (Ser139). |
| Application |
Western Blot: 0.5 µg/ml-2 µg/ml |
| Crossreactivity | All Vertebrates |
| Specificity |
This antibody reacts to Histone H2A.X only when phosphorylated at serine 139. No cross reactivity with other phosphorylated histones. |
| Purity | Protein A purified. |
| Purity Detail | Protein A affinity purified from an animal origin-free culture supernatant. |
| Concentration | 1 mg/ml |
| Formulation | Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. |
| Isotype Negative Control | |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| Accession Number | P16104 |
| Declaration | Manufactured by RevMab Biosciences. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
Histones are proteins that package DNA into nucleosomes. Histones are responsible for maintaining the shape and structure of a nucleosome. One chromatin molecule is composed of at least one of each core histones per 100 base pairs of DNA. There are five families of histones known to date; these histones are termed H1/H5, H2A, H2B, H3, and H4. H2A is considered a core histone, along with H2B, H3 and H4. Core formation first occurs through the interaction of two H2A molecules. Then, H2A forms a dimer with H2B; the core molecule is complete when H3-H4 also attaches to form a tetramer. Histone H2A is composed of non-allelic variants, including H2A.1, H2A.2, H2A.X, and H2A.Z. H2A packages DNA molecules into chromatin and has been correlated with DNA modification and epigenetics. H2A plays a major role in determining the overall structure of chromatin and regulates gene expression. Protein modification on histone H2A exist and can sometimes result in a change in function. Different H2A variants were exploited to have different functions, genetic sequences and modifications.