RevMab

5-Methylcytosine (5-mC) DNA Matched Antibody Pair

CHF 777.00

In stock

REV-31-1110-MK-KI011 SetCHF 777.00

Figure 1: Detection of 5-Methylcytosine in mouse different tissues genomic DNA. Sandwich ELISA using anti-5-Methylcytosine (clone RM231) as the capture antibody (50ng/well at 1ug/mL), and biotinylated anti-Adenine and Guanine (clone RM476) as the detection antibody (0.2ug/mL), followed by an AP-conjugated streptavidin.

Specifications / Handling

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Product Details
Synonyms	5-mC
Product Type	Set
Properties
Application Set	Quantitative ELISA
Specificity	This antibody pair detects 5-methylcytosine in denatured DNA (single strand DNA). It does not react to non-methylated cytosine and other modified cytosine (5-hmC, 5-caC, or 5-fC) in DNA. This product includes capture and detection antibodies for Sandwich ELISA assays.
Crossreactivity	All
Quantity	ELISA: Capture Antibody 50 µg at 1.0mg/mL Detection Antibody 25 µg at 1.0mg/mL
Assay Type	Sandwich
Detection Type	Colorimetric
Kit Contains	1) Capture Antibody: Anti-5-Methylcytosine Rabbit Monoclonal Antibody, Clone RM231, 50 ug at 1.0 mg/mL in 50% Glycerol/PBS with 0.09% sodium azide, without BSA or other carrier proteins. 2) Detection Antibody: Biotin Anti-Adenine and Guanine Rabbit Monoclonal Antibody, Clone RM476, 25 ug at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide;
Other Product Data	Click here for Original Manufacturer Product Datasheet Our product description may differ slightly from the original manufacturers product datasheet.
Declaration	Manufactured by RevMab Biosciences.
Shipping and Handling
Shipping	BLUE ICE
Long Term Storage	-20°C
Handling Advice	Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.
Documents
Manual	No
MSDS	Inquire
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

5-Methylcytosine is a methylated form of the DNA base cytosine that regulates gene transcription and is involved in epigenetics. It is one of the best known epigenetic modifications. This methylation typically occurs at cytosine in CpG dinucleotides in vertebrates. When cytosine is methylated, the DNA maintains the same sequence, but the expression of methylated genes can be altered. 5-Methylcytosine is a very important repressor of transcription in the genome. When present in promoters, 5-Methylcytosine is associated with stable, long-term transcriptional silencing. This may occur by either blocking positive transcription factors, or promoting the binding of negative ones.

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