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RevMab
anti-5-Hydroxymethylcytosine, Rabbit Monoclonal (RM236)
600
CHF
CHF 600.00
In stock
REV-31-1111-00-C05050 µgCHF 600.00
Figure 1: Immunohistochemical staining of formalin fixed and paraffin embedded human brain tissue sections
| Product Details | |
|---|---|
| Synonyms | 5-hmC |
| Product Type | Recombinant Antibody |
| Properties | |
| Clone | RM236 |
| Isotype | Rabbit IgG |
| Source/Host | Rabbit |
| Immunogen/Antigen | BSA-conjugated 5-hydroxymethylcytosine. |
| Application |
DotBlot: 0.2 µg/ml-1 µg/ml |
| Crossreactivity | All Vertebrates |
| Specificity |
This antibody reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA. |
| Purity | Protein A purified. |
| Purity Detail | Protein A affinity purified from an animal origin-free culture supernatant. |
| Concentration | 1 mg/ml |
| Formulation | Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. |
| Isotype Negative Control | |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| Accession Number | N/A |
| Declaration | Manufactured by RevMab Biosciences. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
5-Hydroxymethylcytosine (5hmC) is the first oxidative product in the active demethylation of 5-methylcytosine (5mC). It not only marks active demethylation but also serves as a relatively stable DNA mark that plays distinct epigenetic roles. It is potentially important in epigenetics, because the hydroxymethyl group on the cytosine can possibly switch a gene on and off. 5hmC is abundantly expressed, but the levels vary significantly depending on the cell type. The highest levels are found in neuronal cells of the central nervous system.