RevMab

anti-Acetyl-Histone H2B (Lys20), Rabbit Monoclonal (RM235)

CHF 251.00
In stock
REV-31-1113-00-C02525 µgCHF 251.00
REV-31-1113-00-C100100 µgCHF 559.00
More Information
Product Details
Synonyms H2B K20ac
Product Type Recombinant Antibody
Properties
Clone RM235
Isotype Rabbit IgG
Source/Host Rabbit
Immunogen/Antigen An acetyl-peptide corresponding to Acetyl-Histone H2B (Lys20).
Application

Western Blot: 0.5 µg/ml-2 µg/ml
ELISA: 0.2 µg/ml-1 µg/ml
Multiplex: 0.1 µg/ml-0.5 µg/ml
Immunocytochemistry: 1 µg/ml- 2 µg/ml

Crossreactivity All Vertebrates
Specificity

This antibody reacts to Histone H2B acetylated at Lysine 20 (K20ac). No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.

Purity Protein A purified.
Purity Detail Protein A affinity purified from an animal origin-free culture supernatant.
Concentration 1 mg/ml
Formulation Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide.
Isotype Negative Control

Rabbit IgG

Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

Accession Number P33778
Declaration Manufactured by RevMab Biosciences.
Shipping and Handling
Shipping BLUE ICE
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF
Description

Histones are proteins that package DNA into nucleosomes. Histones are responsible for maintaining the shape and structure of a nucleosome. One chromatin molecule is composed of at least one of each core histones per 100 base pairs of DNA. There are five families of histones known to date; these histones are termed H1/H5, H2A, H2B, H3, and H4. H2B is considered a core histone, along with H2A, H3 and H4. H2A packages DNA molecules into chromatin and has been correlated with DNA modification and epigenetics. H2A plays a major role in determining the overall structure of chromatin and regulates gene expression. Protein modification on histone H2A exist and can sometimes result in a change in function. Different H2A variants were exploited to have different functions, genetic sequences and modifications. Histone H2B is a structural protein that helps organize eukaryotic DNA. It plays an important role in the biology of the nucleus where it is involved in the packaging and maintaining of chromosomes, regulation of transcription and replication and repair of DNA. Histone H2B helps regulate chromatin structure and function through post-translational modifications and specialized histone variants. Hyperacetylation of histone tails helps DNA-binding proteins access chromatin by weakening histone-DNA and nucleosome-nucleosome interactions. Acetylation of a specific lysine residue binds to bromine-containing domains of certain transcription and chromatin regulatory proteins. This docking facilitates the recruitment of these proteins to the correct region of the chromosome. Ubiquitinated histone H2B is often found in regions of active transcription and stimulates transcriptional elongation and sets the stage for further modifications that regulate multiple elements of transcription. In histone H2B, a phosphorylated serine or threonine residue activates transcription.

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