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RevMab
anti-Phospho-eIF4E (Ser209), Rabbit Monoclonal (RM452)
459
CHF
CHF 459.00
In stock
REV-31-1344-00-R100100 µlCHF 459.00
Figure 1: Western Blot of the lysates of MCF-7 cells nontreated (-) or treated (+) with fetal bovine serum, using anti-Phospho-eIF4E (Ser209) rabbit monoclonal antibody (clone RM452) at a 1:1000 dilution.
| Product Details | |
|---|---|
| Synonyms | Eukaryotic Translation Initiation Factor 4E |
| Product Type | Recombinant Antibody |
| Properties | |
| Clone | RM452 |
| Isotype | Rabbit IgG |
| Source/Host | Rabbit |
| Immunogen/Antigen | A phospho-peptide corresponding to human Phospho-eIF4E (Ser209). |
| Application |
Western Blot (WB): 1:1000-1:2000 |
| Crossreactivity | Human |
| Specificity |
This antibody reacts to human eIF4E only when phosphorylated at Ser209. There is no cross-reactivity to eIF4E that is not phosphorylated. This antibody may also react to mouse or rat Phospho-eIF4E (Ser209) as predicted by immunogen homology. |
| Purity | Protein A purified. |
| Purity Detail | Protein A affinity purified from an animal origin-free culture supernatant. |
| Concentration | N/A |
| Formulation | Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. |
| Isotype Negative Control | |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| Accession Number | P06730 |
| Declaration | Manufactured by RevMab Biosciences. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
eIF4E is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). eIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of the eIF4F pre-initiation complex. Almost all cellular mRNA require eIF4E in order to be translated into protein. The eIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis.