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RevMab
anti-Phospho-Myl2 (Myosin Light Chain 2v) (Ser19), Rabbit Monoclonal (RM484)
459
CHF
CHF 459.00
In stock
REV-31-1376-00-R100100 µlCHF 459.00
Figure 1: Western Blot of mouse heart tissue lysate, using Anti-Phospho-Myl2 (Ser19) rabbit monoclonal antibody (clone RM484) at a 1:1000 dilution. The phospho-specificity of RM484 was verified by peptide blocking using a control non-phosphopeptide or phosphopeptide targeting residue Ser19.
| Product Details | |
|---|---|
| Synonyms | Myosin Regulatory Light Chain 2; Ventricular/cardiac Muscle Isoform, MLC-2v |
| Product Type | Recombinant Antibody |
| Properties | |
| Clone | RM484 |
| Isotype | Rabbit IgG |
| Source/Host | Rabbit |
| Immunogen/Antigen | A phospho-peptide corresponding to human Phospho-Myl2 (Ser19). |
| Application |
Western Blot (WB): 1:1000-1:2000 dilution |
| Crossreactivity |
Human Mouse Rat |
| Specificity |
This antibody reacts to Myl2 (Myosin Light Chain 2v) only when phosphorylated at Ser19. There is no crossreactivity to Myl2 that is not phosphorylated. This antibody reacts to human, mouse or rat Phospho-Myl2 (Ser19). |
| Purity | Protein A purified. |
| Purity Detail | Protein A affinity purified from an animal origin-free culture supernatant. |
| Concentration | N/A |
| Formulation | Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. |
| Isotype Negative Control | |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| Accession Number | P10916 |
| Declaration | Manufactured by RevMab Biosciences. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
Myosin is composed of six polypeptide chains: two identical heavy chains and two pairs of light chains. Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC or RLC, LC20), has many isoforms depending on its distribution. In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+/calmodulin-dependent manner. The phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction. ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fiber.