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RevMab
anti-IgG (rabbit), Goat Monoclonal (RMG03) (Biotin)
516
CHF
CHF 516.00
In stock
REV-42-1030-02-C05050 µgCHF 516.00
Figure 1: ELISA of IgGs from different species shows RMG03 reacts to rabbit IgG; no cross reactivity with human IgG, rat IgG, or mouse IgG. The plate was coated with 50ng/well of different IgG. 500 ng/mL, 200 ng/mL, or 50ng/mL of RMG03 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
| Product Details | |
|---|---|
| Synonyms | Immunoglobulin G |
| Product Type | Recombinant Antibody |
| Properties | |
| Clone | RMG03 |
| Isotype | Goat IgG |
| Source/Host | Goat |
| Immunogen/Antigen | Rabbit IgG. |
| Label/Conjugates | Biotin |
| Application |
ELISA: 0.01 µg/ml-0.5 µg/ml Immunocytochemistry (ICC): 0.5 µg/ml-2 µg/ml Immunohistochemistry (IHC): 0.5 µg/ml-2 µg/ml Western Blot (WB): 0.1 µg/ml-0.5 µg/ml |
| Crossreactivity | Rabbit |
| Specificity | This antibody reacts to Rabbit IgG. No cross reactivity with human IgG, rat IgG, or mouse IgG. |
| Purity | Protein A purified. |
| Purity Detail | Protein A affinity purified from an animal origin-free culture supernatant. |
| Concentration | 1 mg/ml |
| Formulation | Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| Accession Number | N/A |
| Declaration | Manufactured by RevMab Biosciences. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
Immunoglobulin G (IgG), a monomer, is the predominant Ig class present in serum. Produced as part of the secondary immune response to an antigen, this class of immunoglobulin constitutes approximately 75% of total serum Ig. Rabbits only have one IgG subclass with two κ light chain types K1 and K2. Because of its relative abundance and excellent specificity toward antigens, IgG is the principle antibody used in immunological research and clinical diagnostics. The common rabbit IgG isotype has a molecular weight of 150 KD, with two heavy chains (about 50 KD each) and two light chains (about 25 KD each) under the non-reducing condition. This anti-rabbit secondary antibody has well-characterized specificity for rabbit IgG immunoglobulins and is useful in the detection, sorting or purification of its specified target. In general, secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody.