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SouthBayBio
UbcH5c [UBE2D3] (human) (rec.) (untagged)
345
CHF
CHF 345.00
In stock
SBB-CE0019-C100100 µgCHF 345.00
Figure 1: Thioester Activity Assay: UBE2D3 (UbcH5c) forms a thioester with Ub in an ATP-dependent manner and the bond can be reduced with addition of access DTT. Confirms the activity of UBE2D3.
| Product Details | |
|---|---|
| Synonyms | Ubiquitin-conjugating Enzyme H5c; PUBC; UBC5C; Ubiquitin-protein Ligase D3; p53-regulated Ubiquitin-conjugating Enzyme |
| Product Type | Protein |
| Properties | |
| Source/Host | E. coli |
| Sequence | Human UBE2D3 (aa1-147) (Accession Nr. P61077) is untagged. |
| Crossreactivity | Human |
| Application | Typical working concentration range is 1-5µM. |
| MW | ~17kDa |
| Purity | ≥95% (SDS-PAGE) |
| Concentration | Lot dependent. |
| Accession Number | P61077 |
| Formulation | Liquid. In 50mM HEPES pH 8, 200mM sodium chloride, 10% glycerol (v/v), 1mM TCEP. |
| Other Product Data |
Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer Our product description may differ slightly from the original manufacturers product datasheet. |
| Declaration | Manufactured by South Bay Bio. |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -80°C |
| Long Term Storage | -80°C |
| Handling Advice | Aliquot to avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -80°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
UBE2D3 [UbcH5c] is an E2 ubiquitin conjugating enzyme. An E1 activating enzyme is required to attach ubiquitin to UBE2D3 via an active site cysteine. The mechanism of ubiquitin transfer involves the breaking of a E1-Ub thioester linkage, followed by a reformation of a UBE2D3-Ub thioester. UBE2D3 is capable of associating with numerous known E3 ligases including MDM2, which target proteins such as p53 for proteasomal degradation through polyubiquitination.