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SouthBayBio
UBE3A (E3 Ligase) (human) (rec.) (His)
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Figure 1: UBE3A SDS-PAGE. From left to right, increasing amounts of protein were loaded onto a 4-20% SDS-PAGE gel, stained with Coomassie brillant blue. Purity is > 98%.
| Product Details | |
|---|---|
| Synonyms | Ubiquitin-protein Ligase E3A; E6AP Ubiquitin-protein Ligase; HECT-type Ubiquitin Transferase E3A; Human Papillomavirus E6-associated Protein; Oncogenic Protein-associated Protein E6-AP |
| Product Type | Protein |
| Properties | |
| Source/Host | Sf9 cells |
| Sequence |
Recombinant human UBE3A (Accession Nr. Q05086) has a C-terminal 8x His-Tag. |
| Crossreactivity | Human |
| MW | ~101kDa |
| Purity | ≥98% (SDS-PAGE) |
| Concentration | Lot dependent. |
| Accession Number | Q05086 |
| Formulation | Liquid. In 50mM HEPES pH 7.3, 100mM NaCl, 100µM TCEP. |
| Other Product Data |
Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer Our product description may differ slightly from the original manufacturer's product datasheet. |
| Declaration | Manufactured by South Bay Bio. |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -80°C |
| Long Term Storage | -80°C |
| Handling Advice | Aliquot to avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -80°C. |
| Documents | |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
UBE3A (Ubiquitin-protein ligase E3A) is a 101 kDa E3 Ligase enzyme. The protein contains a C4 type zinc-finger and belongs to the HECT-type E3 ligase family with its active site cystein located at aa 843. UBE3A is involved in multiple diseases. It is used by the oncoprotein E6 (HPV) to trigger p53 degradation, enabling cervical cancer. It appears to be involved in metastatic prostate cancer, linked to Angelman Syndrome, and elevated expression is correlated with autism disorders. Furthermore, recent findings revealed a high-affinity binding site RAZUL (Rpn10 AZUL-binding domain), with the proteasome and with Rpn10 (PSMD4), prompting its involvement in the ubiquitination of many proteins destined for 26S proteasome degradation. Knockdown of UBE3A leads to a significant reduction in ubiquitinated proteins at the proteasome. It is hypothesized that UBE3A may belong to a subset of E3 ligases prioritized by the proteasome. This recombinant protein (human) has been expressed and purified from Sf9 insect cells and has a C-terminal 8x His-Tag.