NEDP1 [SENP8] (human) (rec.) (His)
SBB-DE0025-C05050 µgCHF 155.00
Michaelis–Menten Kinetics: NEDD8 Rhodamine 110 serially diluted from 1.6 to 0.0125µM was digested with 30pM NEDP1 over time. The assay was carried out in a reaction buffer of 50mM HEPES pH 7.5, 1mM TCEP, 0.1 mg/ml BSA, at 25°C. Initial velocities at each substrate concentration were plotted and fit to the Michaelis–Menten equation. Kinetic parameters were calculated at: Km= 0.087μM, Vmax = 4.44nMs-1, kcat= 148s-1, kcat/Km= 1.70 x 109M-1s-1.
|Synonyms||NEDD8-specific Protease 1; Sentrin-specific Protease 8; Deneddylase-1; Sentrin/SUMO-specific Protease SENP8; DEN1; PRSC2|
|Sequence||Human NEDP1 (aa2-212) (Accession Nr. Q96LD8) is fused at the N-terminus to a His-tag.|
|Application||Typical working concentration range is 1-10nM.|
|Formulation||Liquid. In 50mM HEPES pH 7.5, 100mM sodium chloride, 1mM TCEP.|
|Other Product Data||
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|Declaration||Manufactured by South Bay Bio.|
|Shipping and Handling|
|Short Term Storage||-80°C|
|Long Term Storage||-80°C|
|Handling Advice||Aliquot to avoid freeze/thaw cycles.|
|Use/Stability||Stable for at least 1 year after receipt when stored at -80°C.|
|Product Specification Sheet|
NEDP1 is a deconjugating enzyme with specificity towards NEDDylated substrates. NEDP1 discriminates between ubiquitinated and NEDDylated targets, despite similar C-terminal regions. NEDP1 is a protease which hydrolyzes the isopeptide bond between the C-terminus of NEDD8 and the respective lysine of the substrate protein to which the NEDD8 is covalently bound. NEDP1 is important in regulating cullin associated ubiquitin ligase enzymes such as those of the SCF (Skp-Cullin-Fbox) family and subsequently, it is important in regulating cell cycle progression and other biological functions.