NEDD8 (human) (rec.) (Rhodamine 110)

CHF 330.00
In stock
SBB-PS0003-C05050 µgCHF 330.00
 
More Information
Product Details
Synonyms Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8; Neddylin; Rub1
Product Type Protein
Properties
Source/Host E. coli
Sequence Human NEDD8 (aa1-76) (Accession Nr. Q15843) conjugated at the C-terminus to a quenched Rhodamine 110 dye.
Crossreactivity Human
Application Protein-based substrate. Typical working range is 50-500nM. Hydrolysis of the conjugate results in fluorescence observable by excitation at 485nm and emission at 535nm.
Label/Conjugates Rhodamine
MW ~9kDa
Purity ≥97% (LCMS)
Concentration Lot dependent.
Accession Number Q15843
Formulation Liquid. In 50mM MES pH 6.0, 150mM sodium chloride.
Other Product Data Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer
Our product description may differ slightly from the original manufacturers product datasheet.
Declaration Manufactured by South Bay Bio.
Shipping and Handling
Shipping DRY ICE
Short Term Storage -80°C
Long Term Storage -80°C
Handling Advice Aliquot to avoid freeze/thaw cycles.
Protect from light.
Use/Stability Stable for at least 1 year after receipt when stored at -80°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF
NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8) is a ubiquitin like protein that plays an important role in regulating development and the cell cycle. NEDD8 is conjugated to a target protein via a signaling cascade similar to ubiquitin: a NEDD8 specific E1 activating enzyme (APPBP1/UBA3) adenylates the C-terminus of NEDD8 which is than subsequently passed to an E2 conjugating enzyme (UBE2M or UBE2F) that facilitates covalent attachment of NEDD8 to a cullin subunit of an SCF E3 ubiquitin ligase. NEDD8-Rhodamine 110 can be used as a substrate for enzymes exhibiting deNEDDylating activity, e.g UCH-L3, UCH-L1, COP9 Signalosome and NEDP1.
© 2017 Adipogen Life Sciences. Pictures: © 2012 Martin Oeggerli. All Rights Reserved.