SouthBayBio

Cullin4a/Rbx1/DDB1/CRBN E3 Ligase Complex TR-FRET Kit

CHF 1'250.00
In stock
SBB-KF0120-KI011 KitCHF 1'250.00
More Information
Product Details
Product Type Kit
Properties
Application Set Compound Screening
Specificity

Readout: Endpoint/Kinetic
Label or Dye: Europium-Cryptate & Cy5
Detection Method: TR-FRET
Substrate Properties: Protein-based Substrate. Typical experimental concentration is 1x.
Excitation/Emission (nm): 304/620 & 304/665

Crossreactivity Human
Quantity

Sufficient Reagents for:
400 x 30μl reactions

Kit Contains

100x UBA1 (150μl)
100x UBE2D1 (150μl)
100x Cul4a/Rbx1 Complex (neddyl.) (150μl)
100x DDB1/CRBN (150μl)
10x Reaction Buffer (1ml)
10x Mg-ATP (1ml)
1 x 384-well white low-volume microplate

Other Product Data

The kit uses ubiquitin labeled with either Europium-Cryptate or Cy5 as FRET pair donor and acceptor fluorophores respectively, completely eliminating the need for antibody based detection setups. Enzymatic incorporation of the labeled ubiquitins into chains conjugated onto CRBN (or substrates) leads to an increase in fluorescence emission at 665nm (Em-Acceptor) and decrease at emission wavelength 620nm (Em-Donor).

Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer
Our product description may differ slightly from the original manufacturers product datasheet.

Declaration Manufactured by South Bay Bio.
Shipping and Handling
Shipping DRY ICE
Short Term Storage -20°C
Long Term Storage -80°C
Handling Advice Aliquot to avoid freeze/thaw cycles.
Avoid freeze/thaw cycles.
Documents
Manual No
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF
Description

South Bay Bio’s Cullin4a/Rbx1/DDB1/CRBN Neddylated E3 Ligase TR-FRET Kit provides a fast and sensitive method monitoring ubiquitin conjugation onto the E3 complex in solution, resulting from an enzymatic ubiquitin cascade without the need of running and staining an SDS gel. The kit enables continuous TR-FRET detection of ubiquitin chain formation onto CRBN (or substrates) in a real-time detection setup, or in an end-point configuration if desired. TR-FRET uses the extended fluorescence emission decay lifetimes typical of rare-earth lanthanides to impart a short time-delay between FRET donor excitation and emission. This delay provides a means to separate “true” signal from short-lived background fluorescence, and reduce interference from compound fluorescence and other assay artifacts.

© 2017 Adipogen Life Sciences. Pictures: © 2012 Martin Oeggerli. All Rights Reserved.