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SouthBayBio
20S Immunoproteasome (human) (untagged)
507
CHF
CHF 507.00
In stock
SBB-PP0004-C02525 µgCHF 507.00
Figure 1: 20S Immunoproteasome vs. 20S Constitutive Proteasome Activity: 20S immunoproteasome is most active against Suc-LLVY-AMC (SBB-PS0010), Ac-PAL-AMC (SBB-PS0007), and Ac-ANW-AMC (SBB-PS0009) substrates, representing physiologically relevant chymotrypsin-like, β1i, and β5i immunoproteasome activity respectively.
| Product Details | |
|---|---|
| Product Type | Protein |
| Properties | |
| Source/Host | Human PBMCs |
| Sequence |
Human 20S Immunoproteasome. |
| Crossreactivity | Human |
| Application |
Experiments should be carried out at 20S immunoproteasome concentrations between 2-5nM. The 20S immunoproteasome is commonly associated with the 19S, PA28 α/β, or the PA28γ regulatory complexes. If choosing to omit PA28 during use, 20S must be chemically activated by addition of 0.035%SDS in final assay buffers. |
| MW | ~700kDa |
| Purity | ≥95% (SDS-PAGE) |
| Concentration | Lot dependent. |
| Formulation | Liquid. In 50mM HEPES pH 7.5, 100mM sodium chloride, 1mM TCEP. |
| Other Product Data |
Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer |
| Declaration | Manufactured by South Bay Bio. |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -80°C |
| Long Term Storage | -80°C |
| Handling Advice | Aliquot to avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -80°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
The 20S immunoproteasome is structurally similar to constitutive 26S proteasome. The 20S core of immunoproteasome contains two outer rings composed of α-subunits, and two internal 7-subunit containing rings each possessing 3 specific subunits responsible for proteasome catalytic activity. In immunoproteasomes these subunits (ß1, ß2, ß5) are replaced by three inducible subunits: PSMB9, PSMB10 and PSMB8, (ß1i, ß2i, ß5i). These stress-induced subunits allow for the production of MHC-1 associating peptides, which are displayed as antigens on the cell surface. These displayed peptides can then be recognized by immune surveillance CD8 T cells. 20S immunoproteasome is recognized as a strong drug target for autoimmune disease and cancer.
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