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SouthBayBio
20S Proteasome (human) (untagged)
278
CHF
CHF 278.00
In stock
SBB-PP0005-C05050 µgCHF 278.00
Figure 1: 20S Immunoproteasome vs. 20S Constitutive Proteasome Activity: 20S Proteasome is most active against Suc-LLVY-AMC (SBB-PS0010), Z-LLE-AMC (SBB-PS0006), and Ac-WLA-AMC (SBB-PS0008) substrates, representing physiologically relevant chymotrypsin-like, β1c, and β5c proteasome activity respectively.
| Product Details | |
|---|---|
| Product Type | Protein |
| Properties | |
| Source/Host | Human Erythrocytes |
| Sequence |
Human 20S Proteasome. |
| Crossreactivity | Human |
| Application |
Able to proteolytically degrade substrates in an ATP-independent manner. The 20S core can be activated chemically with SDS (0.035%) or by the addition of PA28. Initial experiments should be carried out at 20S proteasome concentrations between 1-5nM. |
| MW | ~700kDa |
| Purity | ≥95% (SDS-PAGE) |
| Concentration | Lot dependent. |
| Formulation | Liquid. In 50mM HEPES pH 7.6, 100mM sodium chloride, 1mM DTT. |
| Other Product Data |
Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer |
| Declaration | Manufactured by South Bay Bio. |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -80°C |
| Long Term Storage | -80°C |
| Handling Advice | Aliquot to avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -80°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
The ubiquitin-proteasome pathway is the major proteolytic system in eukaryotic cells, where it catalyzes the selective degradation of short-lived regulatory proteins or the rapid turnover of misfolded proteins. One of the most important proteases in this pathway is the 26S proteasome, an ATP-dependent proteolytic complex, which is formed by the association of the barrel-shaped 20S proteasome (700kDa) and two 19S (700kDa) regulatory complexes. The 20S catalytic core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 α-subunits and 2 rings are composed of 7 β-subunits. The 20S catalytic core is able to degrade a variety of peptide substrates and poly-ubiquitinated proteins involved with apoptosis, DNA repair, endocytosis and cell cycle control.
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