Cookie Policy: This site uses cookies to improve your experience. You can find out more about our use of cookies in our Privacy Policy. By continuing to browse this site you agree to our use of cookies.
SouthBayBio
NEDD8 (human) (rec.) (Rhodamine 110)
434
CHF
CHF 434.00
In stock
SBB-PS0003-C05050 µgCHF 434.00
Figure 1: Signal to Background: The signal to background ratio was determined by 100% hydrolysis of 800nM, 400nM, 200nM, 100nM, 50nM NEDD8-Rhodamine 110 to liberate the quenched conjugate. Assay Buffer: 50mM HEPES pH7.5, 1mM TCEP, 0.1mg/ml BSA.
Figure 2: Robustness of NEDD8-Rhodamine 110 substrate: 400nM of NEDD8-Rhodamine 110 was incubated with and without 30pM NEDP1 in a 384 well plate (n=16), and progress curves were normalized to the maximum fluorescence signal to produce “% reaction progress”. The Z’ value, a statistical parameter widely used in the evaluation of screening assays, was calculated at each time-point.
| Product Details | |
|---|---|
| Synonyms | Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8; Neddylin; Rub1 |
| Product Type | Protein |
| Properties | |
| Source/Host | E. coli |
| Sequence | Human NEDD8 (aa1-76) (Accession Nr. Q15843) conjugated at the C-terminus to a quenched Rhodamine 110 dye. |
| Crossreactivity | Human |
| Application | Protein-based substrate. Typical working range is 50-500nM. Hydrolysis of the conjugate results in fluorescence observable by excitation at 485nm and emission at 535nm. |
| Label/Conjugates | Rhodamine |
| MW | ~9kDa |
| Purity | ≥97% (LCMS) |
| Concentration | Lot dependent. |
| Accession Number | Q15843 |
| Formulation | Liquid. In 50mM MES pH 6.0, 150mM sodium chloride. |
| Other Product Data |
Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer Our product description may differ slightly from the original manufacturers product datasheet. |
| Declaration | Manufactured by South Bay Bio. |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -80°C |
| Long Term Storage | -80°C |
| Handling Advice |
Aliquot to avoid freeze/thaw cycles. Protect from light. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -80°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8) is a ubiquitin like protein that plays an important role in regulating development and the cell cycle. NEDD8 is conjugated to a target protein via a signaling cascade similar to ubiquitin: a NEDD8 specific E1 activating enzyme (APPBP1/UBA3) adenylates the C-terminus of NEDD8 which is than subsequently passed to an E2 conjugating enzyme (UBE2M or UBE2F) that facilitates covalent attachment of NEDD8 to a cullin subunit of an SCF E3 ubiquitin ligase. NEDD8-Rhodamine 110 can be used as a substrate for enzymes exhibiting deNEDDylating activity, e.g UCH-L3, UCH-L1, COP9 Signalosome and NEDP1.
Related Products
