SUMO1 (human) (rec.) (Rhodamine 110)

CHF 434.00
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SBB-PS0028-C05050 µgCHF 434.00
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Product Details
Synonyms Small Ubiquitin-related Modifier 1; GAP-modifying Protein 1; SMT3C, SMT3H3, UBL1; Sentrin; Ubiquitin-homology Domain Protein PIC1
Product Type Protein
Source/Host E. coli
Sequence Human SUMO1 (aa1-97) (Accession Nr. P63165) conjugated at the C-terminus to a quenched Rhodamine 110 dye.
Crossreactivity Human
Application Protein-based substrate. Typical working concentration range is 50-500nM. Hydrolysis of the conjugate results in fluorescence observable by excitation at 485nm and emission at 535nM.
Label/Conjugates Rhodamine
MW ~12kDa
Purity ≥97% (LCMS)
Concentration Lot dependent.
Accession Number P63165
Formulation Liquid. In 50mM HEPES pH 7.5, 100mM sodium chloride.
Other Product Data Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer
Our product description may differ slightly from the original manufacturers product datasheet.
Declaration Manufactured by South Bay Bio.
Shipping and Handling
Shipping DRY ICE
Short Term Storage -80°C
Long Term Storage -80°C
Handling Advice Aliquot to avoid freeze/thaw cycles.
Protect from light.
Use/Stability Stable for at least 1 year after receipt when stored at -80°C.
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF

SUMO1 is a ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. This SUMO1 substrate is C-terminally derivatized with a bis-Gly-Rhodamine 110 fluorophore. The bis-Gly-Rh110 is quenched until the amide bond between the C-terminal glycine and the bis-Gly-Rh110 compound is hydrolyzed to mono-Gly-Rhodamine 110. The efficiency of quenching combined with the powerful signal upon hydrolysis yields a reagent with unparalleled signal-to-background. SUMO1-Rh110 can be used to study the deSUMOylating activity of hydrolases SENP1 and SENP2, or other deSUMOylating enzymes. The substrate activity of SUMO1-Rhodamine 110 was determined by measuring the SENP1 catalyzed release of unquenched mono-Gly-Rh110.

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