SouthBayBio

Ubiquitin-AMC (human) (rec.) (untagged)

CHF 291.00
In stock
SBB-PS0043-C05050 µgCHF 291.00
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Product Details
Synonyms Ub-AMC; Ub-7-Amido-4-methylcoumarin
Product Type Protein
Properties
Source/Host E. coli
Sequence

Full-length, mature ubiquitin polypeptide (amino acids 1-76) (Accession Nr. P0CG47) conjugated on its C-terminus to AMC.

Crossreactivity Human
Application

Hydrolysis of the conjugate results in fluorescence observable by excitation at 345nm and emission at 445nm.  Typical working range is 50-500nM.

Label/Conjugates AMC
MW ~8.7kDa
Purity ≥99% (LCMS)
Concentration Lot dependent.
Accession Number P0CG47
Formulation Liquid. In 50mM MES pH 6.0.
Other Product Data

Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer
Our product description may differ slightly from the original manufacturers product datasheet.

Declaration Manufactured by South Bay Bio.
Shipping and Handling
Shipping DRY ICE
Short Term Storage -80°C
Long Term Storage -80°C
Handling Advice Aliquot to avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -80°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF
Description

Ubiquitin is a 76 amino acid post-translational modifier expressed throughout all tissues in eukaryotic organisms. The many roles of ubiquitin modification include proteasomal degradation, signal transduction, inflammatory response, and DNA damage repair. Ubiquitin modification occurs through a pyramidal cascade of an E1 activating enzyme, E2 conjugating enzymes, and an E3 ubiquitin ligases. This enzymatic cascade results in modification of a ε-amine of a lysine residue on a substrate protein. Substrates may either be mono or poly-ubiquitinated by M1, K6, 11, 27, 29, 33, 48 or 63 linkages. Removal of ubiquitin from a substrate protein occurs via deconjugating enzymes, of which there are nearly 100 known enzymes with various specificities.

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