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SouthBayBio
Ubiquitin-AMC (human) (rec.) (untagged)
291
CHF
CHF 291.00
In stock
SBB-PS0043-C05050 µgCHF 291.00
Figure 1: Robustness of Ubiquitin-AMC substrates in an HTS format: 500nM Ubiquitin-AMC was incubated with and without 5pM UcHL3 in a 384 well plate (n = 16), and progress curves were normalized to the maximum fluorescence signal to produce, i.e. the “% reaction progress”. The Z’ value, a statistical parameter widely used in the evaluation of screening assays, was calculated at each time-point.
Figure 2: Signal to Background: The signal to background ratio was determined by 100% hydrolysis of 50nM Ubiquitin-AMC to liberate the quenched conjugate. Assay Buffer: 50mM HEPES pH7.5, 100mM NaCl, 1mM TCEP, 0.1mg/ml BSA.
| Product Details | |
|---|---|
| Synonyms | Ub-AMC; Ub-7-Amido-4-methylcoumarin |
| Product Type | Protein |
| Properties | |
| Source/Host | E. coli |
| Sequence |
Full-length, mature ubiquitin polypeptide (amino acids 1-76) (Accession Nr. P0CG47) conjugated on its C-terminus to AMC. |
| Crossreactivity | Human |
| Application |
Hydrolysis of the conjugate results in fluorescence observable by excitation at 345nm and emission at 445nm. Typical working range is 50-500nM. |
| Label/Conjugates | AMC |
| MW | ~8.7kDa |
| Purity | ≥99% (LCMS) |
| Concentration | Lot dependent. |
| Accession Number | P0CG47 |
| Formulation | Liquid. In 50mM MES pH 6.0. |
| Other Product Data |
Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer |
| Declaration | Manufactured by South Bay Bio. |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -80°C |
| Long Term Storage | -80°C |
| Handling Advice | Aliquot to avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -80°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
Ubiquitin is a 76 amino acid post-translational modifier expressed throughout all tissues in eukaryotic organisms. The many roles of ubiquitin modification include proteasomal degradation, signal transduction, inflammatory response, and DNA damage repair. Ubiquitin modification occurs through a pyramidal cascade of an E1 activating enzyme, E2 conjugating enzymes, and an E3 ubiquitin ligases. This enzymatic cascade results in modification of a ε-amine of a lysine residue on a substrate protein. Substrates may either be mono or poly-ubiquitinated by M1, K6, 11, 27, 29, 33, 48 or 63 linkages. Removal of ubiquitin from a substrate protein occurs via deconjugating enzymes, of which there are nearly 100 known enzymes with various specificities.
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