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SouthBayBio
Skp1/Skp2 Complex (human) (rec.) (His)
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Figure 1: Skp1/His-Skp2 SDS-PAGE. 1, 2, and 4 μg of Skp1/His-Skp2 were loaded onto a 4-20% SDS-PAGE gel, stained with Coomassie brillant blue. Purity is >95%.
| Product Details | |
|---|---|
| Synonyms | S-phase Kinase-associated Protein 1 / S-phase Kinase-associated Protein 2 |
| Product Type | Protein |
| Properties | |
| Source/Host | Sf9 cells |
| Sequence |
Recombinant human Skp1 (1-163) and Skp2 (1-424) heterodimer. N-terminal His-tag on Skp2. |
| Crossreactivity | Human |
| Application |
Working concentrations for this enzyme complex range from 20-50nM for TR-FRET-based ubiquitination assays in 384 plates. |
| MW | Skp1 ~18.6kDa, Skp2 ~48kDa |
| Purity | ≥95% (SDS-PAGE) |
| Concentration | Lot dependent. |
| Accession Number | P63208: SKP1 / Q13309: SKP2 |
| Formulation | Liquid. In 50mM HEPES pH 7.5, 150mM NaCl, 1mM TCEP. |
| Other Product Data |
Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer Our product description may differ slightly from the original manufacturer's product datasheet. |
| Declaration | Manufactured by South Bay Bio. |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -80°C |
| Long Term Storage | -80°C |
| Handling Advice | Aliquot to avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -80°C. |
| Documents | |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
SKP1 (S-phase kinase-associated protein 1) is an essential component of SCF (SKP1-CUL1-F-box protein) E3 Ubiquitin ligases that mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. SKP1 is the adapter protein that links Cullin-1 to various F-box proteins (such as SKP2) that serve as the substrate recognition components of SCF ligases. SKP2 targets include proteins involved in cell cycle progression and signal transduction. SKP2 recognizes phosphorylated CDKN1B/ p27Kip and is involved in G1-S cell cycle transition in a CKS1-dependent manner. The Skp1/Skp2 comlex is associated with Cullin-1 (CUL1) a core component of multiple SCF (SKP1-CUL1-F-box) E3 Ubiquitin ligase complexes mediating the ubiquitination of proteins. This complex consists of an N-terminal 6x-His tagged Skp2 and an untagged Skp1. The complex has been expressed and purified from insect cells Sf9.