SouthBayBio

TRF-Ubiquitin Mix (100X) (400 reactions)

CHF 1'233.00
In stock
SBB-TR0051-4H-11 VialCHF 1'233.00
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Product Details
Synonyms TRF-Ub Mix
Product Type Protein
Properties
Source/Host E. coli
Crossreactivity Human
Application

TR-FRET: 400 x 20μl reactions or 160 x 50μl reactions
Readout: Endpoint/Kinetic
Label or Dye: Europium Cryptate & Cy5
Detection Method: TR-FRET
Substrate Properties: Protein-Based Substrate. Typical experimental concentration is 1x.
Excitation/Emission (nm): 304/620 & 304/665

MW 8.5 to 8.9 kDa
Purity ≥99% (LCMS)
Formulation Liquid. In 50mM HEPES pH 7.5, 100mM NaCl.
Other Product Data

This product is a mix of Europium-Cryptate Ubiquitin (SBB-TR0014), Cy5-Ubiquitin (SBB-TR0015), and wild-type Ubiquitin (SBB-UP0013) in a ratio optimized for TR-FRET based conjugation experiments where long polyubiquitin chains are formed. Under conditions where short ubiquitin chains are formed, a different mix may be required to optimize signal to background ratio. Enzymatic incorporation of the labeled ubiquitins into chains conjugated onto a substrate protein leads to an increase in fluorescence emission at 665nm (Em-Acceptor) and decrease at emission wavelength 620nm (Em-Donor).

Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer
Our product description may differ slightly from the original manufacturers product datasheet.

Declaration Manufactured by South Bay Bio.
Shipping and Handling
Shipping DRY ICE
Short Term Storage -20°C
Long Term Storage -80°C
Handling Advice Aliquot to avoid freeze/thaw cycles.
Avoid freeze/thaw cycles.
Protect from light.
Use/Stability Stable for at least 1 year after receipt when stored at -80°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF
Description

Ubiquitin is a highly conserved protein that plays a major role in the ubiquitination pathway, which is conserved from yeast to mammals. Ubiquitination, the conjugation of ubiquitin to other proteins through a covalent bond between its C-terminal glycine and the ε-amino group of lysine residues or the α-amino group of an N- terminal methionine onto proteins is essential for many cellular processes primarily linked to protein degradation. This process involves three steps with specific groups of enzymes in an ATP depended manner, which are activation with ubiquitin-activating enzymes (E1s), conjugation with ubiquitin-conjugating enzymes (E2s) and ligation with ubiquitin ligases (E3s).

This product is a mix of Europium Cryptate Ubiquitin (SBB-TR0014), Cy5-Ubiquitin (SBB-TR0015), and wild-type Ubiquitin (SBBUP0013) in a ratio optimized for TR-FRET based conjugation experiments where long polyubiquitin chains are formed. Under conditions where short Ubiquitin chains are formed, a different mix may be required to optimize signal to background ratio. Enzymatic incorporation of the labeled ubiquitins into chains conjugated onto a substrate protein leads to an increase in fluorescence emission at 665nm (Em acceptor) and decrease at emission wavelength 620nm (Em donor).

Product References
  1. Imidodiphosphinate ligands as antenna units in luminescent lanthanide complexes. s. Magennis, et al.; Chemical Communications 1, 61 (1999)
  2. Ubiquitin Ligases: Structure, Function, and Regulation.  N. Zheng & N. Shabek; Annual Review of Biochemistry (2017)
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