anti-Mouse/Rabbit IgG (H+L), made in horse, biotinylated

Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining, ELISAs, and blots. Biotinylated Universal Horse Anti-Mouse/Rabbit IgG (H+L) is supplied in liquid format. With some exceptions the recommended dilution for most applications is 1:50. (H+L) indicates the antibody recognizes both heavy and light chains. This biotinylated Universal secondary antibody is produced in horse and binds equally well to both mouse IgG (H+L) and rabbit IgG (H+L) primary antibodies. The high staining quality and sensitivity achieved using this product is equivalent to that obtained with either our biotinylated anti-mouse IgG (H+L) or biotinylated anti-rabbit IgG (H+L) used individually. The convenience of using a single biotinylated secondary antibody for both species of primary and the elimination of possible mistakes in using the wrong secondary antibody is an advantage of this product. Note: This antibody has significant crossreactivity for rodent IgG and is not recommended for use in staining rodent tissue. This product is the same biotinylated secondary antibody included in the VECTASTAIN Elite ABC Kit (Universal; PK-6200) and VECTASTAIN ABC-AP Kit (Universal; AK-5200), but is not the same as that provided in the VECTASTAIN Universal Quick Kit, PK-8800.

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