ImmPRESS™ Duet Immunofluorescence Double Staining Polymer Kit (rabbit IgG HRP/mouse IgG AP)

15 ml BLOXALL™ Blocking Solution
15 ml 2.5% Normal Serum
15 ml ImmPRESS™ Duet HRP/AP Polymer Reagent (mixture of ImmPRESS™ HRP Anti-Rabbit IgG and ImmPRESS™ AP Anti-Mouse IgG)
ImmPACT™ DAB EqV Substrate (HRP, brown)
ImmPACT™ Vector® Red Substrate (AP, magenta)

The ImmPRESS™ Duet Double Staining Polymer Kit is intended for use on non-rodent tissue specimens. Reliable and reproducible results are obtained on tissue sections where the two target antigens do not over-lap (co-localize) in the same structure of the same cell, but rather are expressed in different cell compartments or different cell types. Save time and streamline your double staining IHC workflow with the ImmPRESS™ Duet Double Staining Polymer Kit. This kit enables fast, well-defined localization and visualization of two different target antigens on the same tissue section. Utilizing our unique ImmPRESS™ micropolymer chemistry, we independently conjugate highly-active horseradish peroxidase (HRP) and alkaline phosphatase (AP) enzyme polymers to anti-mouse IgG and anti-rabbit IgG affinity-purified, highly cross-adsorbed secondary antibodies. These reagents are then blended at optimized dilutions to provide a stable pre-diluted, ready-to-use HRP/AP formula that will detect mouse and rabbit primary antibodies, and facilitate a time-saving, simplified, one-step double label detection protocol. For maximum sensitivity and staining contrast between target antigens, ImmPACT™ DAB EqV (HRP, brown) and ImmPACT™ Vector® Red (AP, magenta) substrates are included to complete this easy to use staining kit.

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