Animal-Free Blocker and Diluent (R.T.U.)

Animal-Free Blocker (SP-5035) is a plant-derived blocking agent and diluent for immunohistochemistry, nucleic acid, protein blotting, and for other applications. This reagent contains no animal-derived protein and can be used as an alternative to sera, BSA, casein, or non-fat dry milk. It is supplied as ready-to-use solution without any sodium azide. As such it can be used with both peroxidase and alkaline phosphatase antibody conjugates and all secondary detection reagents including polymer systems and avidin/biotin reagents that incorporate these enzymes. This makes the blocking solution especially convenient in multiplex (multiple antigen labeling) IHC applications where antibodies from different species and a variety of detection systems are used on the same tissue section. Importantly, this is a different formulation than our concentrated (5x) animal free blocker, due to the optimized conditions and neutral pH of the solution for IHC and IF methods.

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