TurboLink Catalyst Buffer (10X)

TurboLink Catalyst Buffer is used to catalyze SoluLink® bioconjugation reactions, which are based on aromatic hydrazone formation between HyNic-modified biomolecules and 4FB-modified biomolecules. TurboLINK greatly accelerates hydrazone formation-in the presence of TurboLink Catalyst the conjugation reaction is typically complete in 1-2 hours. The active ingredient, aniline, is delivered at 100 mM concentration in Conjugation Buffer. A final concentration of 5-10 mM aniline in the conjugation reaction is sufficient for hydrazone catalysis. This reagent is particularly useful for conjugation of large biomolecules such as antibodies, oligonucleotides, proteins, enzymes, and surfaces.