BioStab Antibody Stabilizer

BioStab Antibody Stabilizer can be used in ELISA, Western Blotting, Immunohistochemistry and any other applications which require marker or biotinylated antibodies. It is supplied as a ready-to-use solution for dilution of antibodies. This stabilizer contains a minimum amount of a surfactant. The stabilizer is free of any protein to minimize potential unspecific interactions. The method below is strongly recommended for standard assay procedures and reduces loss of valuable conjugates and assay-to-assay variations.

Dilution to stock concentration: The concentrated conjugate solution should be diluted to a concentration appropriate to perform titration or other dilution experiments (e. g., 1/1000). This stock can be stored for the indicated time without loss of activity. Dilution can be performed using appropriate additives. Avoid phosphate buffers at concentrations higher than 3mM. Diluted solution should be used immediately. No warranty can be given on the stabilization of diluted stabilizer solutions.

Dilution to assay concentrations: Dilute an amount of antibody needed for the next period (of up to one year) to the desired concentration (e. g., 1:5,000 for ELISA). If desired, add a specific blocking agent. Keep the solution refrigerated until use, then warm up the required amount, pipet directly into the wells in use and perform your assay. The dilution should best be stored refrigerated. Inactivation in the stabilizer is clearly a thermal process and life time can be further increased by applying low temperatures. However, not to disturb the assay performance it is recommended to take the amount needed and bring it to room temperature. Any excess reagent can thereafter be returned to the refrigerator without interference with life time of the dilution.