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AdipoGen Life Sciences
BioStab PCR Optimizer
63
CHF
CHF 63.00
In stock
AG-10T-0032-L0011 mlCHF 63.00
AG-10T-0032-L0055 mlCHF 184.00
Amplification of a 375 bp fragment from the 5' region of mouse cAMP-dependent protein kinase Cβ gene (74% GC) using different annealing temperature: BioStab PCR optimizer allows the use of a wide range of annealing temperatures thereby eliminating the need for an optimization of amplification conditions. Lane 2-4 show the PCR product obtained in the presence of BioStab PCR optimizer at different annealing temperatures, Lane 5-7 show the result of a PCR-reaction without BioStab PCR optimizer. 1 – DNA Ladder.
Amplification of a target with GC-content > 70%: Human retinoblastoma gene contains a region at its 5´-end with a GC-content of 75% and cannot be amplified without special additives to the PCR mixture (lane 2). Addition of the BioStab PCR optimizer to the PCR mixture in the final concentration of 1x allows efficient amplification of the desired 180 bp fragment (lane 3). Addition of either competitor’s product or 1M betaine produces a non-specific band additionally to the desired product (lanes 4 and 5). 1- DNA Ladder.
Amplification of a target with GC-content > 75%: Targets with GC-content higher than 75% may require addition of increased amount of BioStab PCR optimizer. An attempt to amplify a 149-bp region of human genomic DNA containing 78.5% of GC pairs and a CGG trinucleotide repeat in regular PCR buffer without additives leads to multiple nonspecific products (lane 2). The specific product was obtained with 2x concentrated BioStab PCR optimizer (lane 3). Inclusion of either a competitor´s product or 1M betaine resulted in the amplification of non-specific products in addition to the desired product (lanes 4 and 5). 1- DNA Ladder.
| Product Details | |
|---|---|
| Product Type | Chemical |
| Properties | |
| Appearance | Clear, light yellow liquid. |
| Formulation | Filtered through a 0.22 μm filter. It is a 5X concentrated solution in sterile, bidestillated water and contains no proteins or preservatives. |
| Declaration | Manufactured by bitop AG, Dortmund/Germany. |
| Other Product Data |
The BioStab PCR optimizer is designed as a PCR additive which improves the yield and specificity of PCR reaction and allows amplification of difficult targets (e.g. sequences with >70% GC pairs). BioStab PCR optimizer enables the use of a wide range of annealing temperatures thereby eliminating the need for an optimization of amplification conditions. The BioStab PCR optimizer is provided as 5x concentrated solution. Working concentration in PCR mixture is 1x. For targets with >75% GC content a 2x solution is recommended. |
| Smiles | |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice | Do not freeze. |
| Use/Stability | Stable for at least 1 year after receipt when stored at +4°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
PCR is a technique extensively used in different applications in molecular biology, molecular diagnostics and forensics. However, some sequences, for example the 5´-regions of many eukaryotic genes, are difficult to amplify. Incomplete separation of DNA strands results in little or no yield of desired product and multiple non-specific PCR-fragments. BioStab PCR Optimizer solves this problem and outperforms betaine. The mode of action of BioStab PCR optimizer comprises lowering the melting temperature of GC pairs without affecting that of AT pairs.
The BioStab PCR optimizer is designed as a PCR additive which improves the yield and specificity of PCR reaction and allows amplification of difficult targets (e.g. sequences with >70% GC pairs). BioStab PCR optimizer enables the use of a wide range of annealing temperatures thereby eliminating the need for an optimization of amplification conditions. The BioStab PCR optimizer is provided as 5x concentrated solution. Working concentration in PCR mixture is 1x. For targets with >75% GC content a 2x solution is recommended.
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