BioStab Peroxidase Conjugate Stabilizer

BioStab Peroxidase Conjugate Stabilizer can be used in ELISA, Western Blotting, Immunohistochemistry and any other applications which require HRP conjugates. It is supplied as ready-to-use solution for dilution of HRP conjugates. This stabilizer contains a minimum amount of a surfactant and is free of any protein to minimize potential unspecific interactions. A procedure for standard assays reducing loss of conjugates and ensuring assay-to-assay reproducibility:

Storage of working solutions: Dilute an amount of conjugate needed for the next period (of up to one year) to the desired concentration (e. g., 1:50,000 for ELISA). Add a specific blocking agent. Keep the solution refrigerated until use, then warm up the amount required, pipet directly into the wells in use and perform your assay. The dilution should best be stored refrigerated.

Storage of stock solutions: The concentrated conjugate solution should be diluted to a concentration appropriate to perform titration or other dilution experiments (e. g., 1/1000). This stock can be stored for the indicated time without loss of activity. Further dilution can be performed using appropriate additives. Avoid phosphate buffers at concentrations higher than 3 mM. Diluted solution should be used immediately. No warranty can be given on the stabilization of diluted stabilizer solutions.