AdipoGen Life Sciences

anti-Listeria monocytogenes p60, mAb (P6007)

CHF 290.00

In stock

AG-20A-0022-C05050 µgCHF 290.00

AG-20A-0022-C100100 µgCHF 390.00

Figure 1: Western Blot analysis using anti-Listeria monocytogenes, mAb (P6007) (Prod. No. AG-20A-0022) at 1:5000 dilution.
1: Recombinant L. monocytogenes p60.
2: Culture media of L. monocytogenes.

Specifications / Handling

More Information
Product Details
Synonyms	IAP p60 from Listeria Monocytogenes; Invasion-associated Protein p60 from Listeria Monocytogenes
Product Type	Monoclonal Antibody
Properties
Clone	p6007
Isotype	Mouse IgG1κ
Immunogen/Antigen	Recombinant Listeria monocytogenes p60.
Application	ELISA: (direct or indirect: 1:5'000-1:10'000) Western blot: (1:2'000-1:10'000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application.
Crossreactivity	Listeria monocytogenes
Specificity	Recognizes L. monocytogenes p60 protein. Does not cross-react with other Listeria species.
Purity Detail	Protein G-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Mouse IgG1 Isotype Control
RRID	AB_2490104
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Listeria monocytogenes is a intracellular bacterium that is the causative agent of Listeriosis. It is one of the most virulent foodborne pathogens. Listeria species are known to secrete a 60-kDa protein collectively termed p60, which is encoded by the iap (invasion-associated-protein) gene and secreted in large quantites into the growth media. p60 is a highly immunogenic murein hydrolase that is essential for cell division.

Product References

Use of monoclonal antibodies that recognize p60 for the identification of listeria monocytogenes: K.-Y. Yu, et al.; Clin. Diag. Lab. Immunol. 11, 446 (2004)
Listeria monocytogenes-induced cell death inhibits the generation of cell-mediated immunity: E. Theisen & J.D. Sauer; Infect. Immun. 85, e00733-16 (2017)
The VirAB ABC transporter is required for VirR regulation of Listeria monocytogenes virulence and resistance to nisin: Infect. Immun. 86, e00901 (2018)
Listeria hijacks host mitophagy through a novel mitophagy receptor to evade killing: Y. Zhang, et al., Nat. Immunol. 20, 433 (2019)
Listeria-vectored multi-antigenic tuberculosis vaccine protects C57BL/6 and BALB/c mice and guinea pigs against Mycobacterium tuberculosis challenge: Q. Jia, et al.; Nature Commun. Biol. 5, 1388 (2022)

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