AdipoGen Life Sciences

anti-Listeria sp. p60, pAb

CHF 390.00
In stock
AG-25A-0019-C100100 µgCHF 390.00
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Product Details
Synonyms IAP p60 from Listeria monocytogenes; Invasion-associated Protein p60 from Listeria monocytogenes
Product Type Polyclonal Antibody
Properties
Source/Host Rabbit
Immunogen/Antigen Recombinant Listeria spp. p60.
Application

ELISA: (direct and indirect: 1:5’000-1:10’000)
Western Blot: (1:2’000-1:10’000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.

Crossreactivity Listeria sp.
Specificity

Recognizes a wide range of Listeria p60 proteins.

Purity Detail Protein A-affinity purified.
Concentration 1mg/ml
Formulation Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control

Rabbit IgG

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

Listeria monocytogenes is a intracellular bacterium that is the causative agent of Listeriosis. It is one of the most virulent foodborne pathogens. Listeria species are known to secrete a 60-kDa protein collectively termed p60, which is encoded by the "iap" (invasion-associated-protein) gene and secreted in large quantites into the growth media. p60 is a highly immunogenic murein hydrolase that is essential for cell division.

Product References
  1. Use of monoclonal antibodies that recognize p60 for the identification of Listeria monocytogenes: K.-Y. Yu, et al.; Clin. Diag. Lab. Immunol. 11, 446 (2004)
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