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AdipoGen Life Sciences
anti-FOXP3 (mouse), mAb (MF333F)
250
CHF
CHF 250.00
In stock
AG-20A-0025Y-C05050 µgCHF 250.00
AG-20A-0025Y-C100100 µgCHF 360.00
Replaces Item #AG-20A-0025
Figure 1: Immunohistochemical staining of Foxp3+ cells (red) in spleen tissue from 25-week-old BALB-neuT mice using anti-FOXP3 (mouse), mAb (MF333F) (Prod. No. AG-20A-0025Y).
Figure 2: Immunofluorescent imaging of activated mouse CD4+ T cells using anti-FOXP3 (mouse), mAb (MF333F) (Prod. No. AG-20A-0025Y) which recognizes mouse FOXP3 in the nucleus.
Figure 3: Western blot analysis of mouse Foxp3 using anti-Foxp3 (mouse), mAb (MF333F) (Prod. No. AG-20A-0025Y) at 1:2,000 dilution.
1. Recombinant mouse Foxp3 (His-tagged).
2. Transfected mouse Foxp3 full length cell lysate (HEK 293).
3. Mock Transfected HEK293 cell lysate.
4. PHA- treated mouse T cell (CD4+) lysate.
1. Recombinant mouse Foxp3 (His-tagged).
2. Transfected mouse Foxp3 full length cell lysate (HEK 293).
3. Mock Transfected HEK293 cell lysate.
4. PHA- treated mouse T cell (CD4+) lysate.
| Product Details | |
|---|---|
| Synonyms | Forkhead Box Protein P3; Scurfin |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | MF333F |
| Isotype | Rat IgG2aκ |
| Source/Host | Purified from concentrated hybridoma tissue culture supernatant. |
| Immunogen/Antigen | Recombinant mouse FOXP3. |
| Application |
ELISA: (direct or indirect: 1:2'000-1:10'000) |
| Crossreactivity | Mouse |
| Specificity |
Recognizes mouse FOXP3. Detects a band of ~46-49kDa by Western blot. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein G-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives. |
| Isotype Negative Control | |
| Other Product Data |
Uniprot: FOXP3 (mouse) |
| Accession Number | Q99JB6 |
| RRID | AB_2490107 |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
FOXP3 is involved in immune system responses. It functions as the master regulator in the development and function of regulatory T cells. FOX proteins belong to the forkhead/winged-helix family of transcriptional regulators and are presumed to exert control via similar DNA binding interactions during transcription. Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX); also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema.
Product References
- Alternatively activated dendritic cells preferentially secrete IL-10, expand Foxp3+CD4+ T cells, and induce long-term organ allograft survival in combination with CTLA4-Ig: Y.Y. Lan, et al.; J. Immunol. 177, 5868 (2006)
- Dendritic cells expressing transgenic galectin-1 delay onset of autoimmune diabetes in mice: M.J. Perone, et al.; J. Immunol. 177, 5278 (2006)
- Immunosurveillance of Erbb2 carcinogenesis in transgenic mice is concealed by a dominant regulatory T-cell self-tolerance: E. Ambrosino, et al.; Cancer Res. 66, 7734 (2006)
- Regulated compartmentalization of programmed cell death-1 discriminates CD4+CD25+ resting regulatory T cells from activated T cells: G. Raimondi, et al.; J. Immunol. 176, 2808 (2006)
- Antimetastatic activity of a preventive cancer vaccine: P. Nanni, et al.; Cancer Res. 67, 11037 (2007)
- Rapamycin-conditioned dendritic cells are poor stimulators of allogeneic CD4+ T cells, but enrich for antigen-specific Foxp3+ T regulatory cells and promote organ transplant tolerance: H.R. Turnquist, et al.; J. Immunol. 178, 7018 (2007)
- Pretransplant infusion of mesenchymal stem cells prolongs the survival of a semiallogeneic heart transplant through the generation of regulatory T cells: F. Casiraghi, et al.; J. Immunol. 181, 3933 (2008)
- Suppression of autoimmune diabetes by soluble galectin-1: M.J. Perone, et al.; J. Immunol. 182, 2641 (2009)
- Protective role for TLR4 signaling in atherosclerosis progression as revealed by infection with a common oral pathogen: C. Hayashi, et al.; J. Immunol. 189, 3681 (2012)
- Acceleration of diabetes development in CXC chemokine receptor 3 (CXCR3)-deficient NOD mice: Y. Yamada, et al.; Diabetologia 55, 2238 (2012)
- Characteristics of CD4 + CD25 + Foxp3 + regulatory T cells in patients with multiple organ dysfunction syndrome: X. Zhang, et al.; Exp. Ther. Med. 11, 1908 (2016)
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