AdipoGen Life Sciences

anti-FOXP3 (mouse), pAb

CHF 190.00

In stock

AG-25A-0035-C100100 µgCHF 190.00

Figure 1: Western blot analysis using anti-FOXP3 (mouse), pAb (Prod. No. AG-25A-0035) at 1:5'000 dilution.
1: Mouse FOXP3 (His-tagged).
2: Transfected mouse FOXP3 cell lysate (HEK 293).
3: Transfected vector only cell lysate.

Specifications / Handling

More Information
Product Details
Synonyms	Forkhead Box Protein P3; Scurfin
Product Type	Polyclonal Antibody
Properties
Source/Host	Rabbit
Immunogen/Antigen	Recombinant mouse FOXP3.
Application	ELISA: (direct and indirect: 1:2’000-1:10’000) Western Blot: (1:2’000-1:5’000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application. Note: Tested on recombinant proteins and/or target-protein transfected cell lines in ELISA, Western Blot and/or FACS.
Crossreactivity	Mouse
Specificity	Recognizes mouse FOXP3. Detects a band of ~46-48kDa by Western blot.
Purity Detail	Protein A-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Rabbit IgG
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

FOXP3 is involved in immune system responses. It functions as the master regulator in the development and function of regulatory T cells. FOX proteins belong to the forkhead/winged-helix family of transcriptional regulators and are presumed to exert control via similar DNA binding interactions during transcription. Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX); also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema.

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