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AdipoGen Life Sciences
anti-IL-33 (human), mAb (IL33305B)
295
CHF
CHF 295.00
In stock
AG-20A-0041-C05050 µgCHF 295.00
AG-20A-0041-C100100 µgCHF 400.00
Figure 1: Immunohistochemical staining of human tissue using anti-IL-33 (human), mAb (IL33305B) (Prod. No. AG-20A-0041) at 1:100 dilution.
A. Immunoperoxidase staining (cytoplasmic) of formalin-fixed, paraffin-embedded human heart (100x, brown colour).
B. Isotype control with IgG2a (negative control).
A. Immunoperoxidase staining (cytoplasmic) of formalin-fixed, paraffin-embedded human heart (100x, brown colour).
B. Isotype control with IgG2a (negative control).
Figure 2: Inhibition of binding of recombinant human IL-33 (hIL-33) to human ST2 (hST2) in vitro using anti-IL-33 (human), mAb (IL33305B) (Prod. No. AG-20A-0041). An indirect competitive ELISA was performed as follows; 1) coat microtiter plate wells with hST2-Fc (10 ug/ml); 2) add 2 ug/ml of IL-33 with or without a hIL-33 mAb to the wells followed by washing; 3) add anti-FLAG HRP conjugated (1:2,000) to an enzyme; 4) after adding the TMB solution, incubate at RT in the dark for 20 minutes. Immediately read the plate at 450 nm.
Figure 3: Immunoprecipitation of recombinant hIL-33 proteins using anti-IL-33 (human), mAb (IL33305B) (Prod. No. AG-20A-0041). Recombinant hIL-33 Proteins were precipitated. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by Western blot/ECL reaction with anti-FLAG or anti-His.
Figure 4: Western blot analysis of human IL-33 using anti-IL-33 (human), mAb (IL33305B) (Prod. No. AG-20A-0041) at 1:2,000 dilution.
1. Recombinant human IL-33 (His tagged).
2. Recombinant human IL-33 (FLAG®-tagged).
3. Recombinant mouse IL-33 (FLAG®-tagged).
4. Other protein (His tagged, negative control).
1. Recombinant human IL-33 (His tagged).
2. Recombinant human IL-33 (FLAG®-tagged).
3. Recombinant mouse IL-33 (FLAG®-tagged).
4. Other protein (His tagged, negative control).
| Product Details | |
|---|---|
| Synonyms | Interleukin-33; IL-1F11; NF-HEV |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | IL33305B |
| Isotype | Mouse IgG2aκ |
| Immunogen/Antigen | Recombinant human IL-33. |
| Application |
ELISA: (direct or indirect: 1:2'000-1:5'000) |
| Crossreactivity | Human |
| Specificity |
Recognizes human IL-33. Does not cross-react with mouse IL-33. |
| Purity Detail | Protein G-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives. |
| Isotype Negative Control | |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Interleukin-33 (IL-33; HF-NEV; IL-1F11), a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released on cell lysis. The 30kDa human IL33 is converted by CASP1 to a 18kDa protein. IL33 binds to and signals through ST2 (IL1R1) and its stimulation recruits MYD88, IRAK, IRAK4, and TRAF6, followed by phosphorylation of ERK1 (MAPK3)/ERK2 (MAPK1), p38(MAPK14), and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells, and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases, and sepsis.
Product References
- Inhibition of interleukin-33 signaling attenuates the severity of experimental arthritis: G. Palmer, et al.; Arthritis & Rheum. 60, 738 (2009)
- Transcriptomic and genetic studies identify IL-33 as a candidate gene for Alzheimer's disease: J. Chapuis, et al.; Mol. Psychiatry 14, 1004 (2009) (Supplement Information)
- Interleukin-33 is biologically active independently of caspase-1 cleavage: D. Talabot-Ayer, et al.; JBC 284, 19420 (2009)
- Cigarette smoke alters IL-33 expression and release in airway epithelial cells: E. Pace, et al.; Biochim. Biophys. Acta 1842, 1630 (2014)
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