IL-33 (human) (rec.) (untagged)

CHF 150.00
In stock
AG-40B-0038-C01010 µgCHF 150.00
AG-40B-0038-50105 x 10 µgCHF 450.00
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Product Details
Synonyms Interleukin-33; IL-1F11; NF-HEV
Product Type Protein
Properties
Source/Host E. coli
Sequence Human IL-33 (aa 112-270) is untagged.
Crossreactivity Human
Mouse
Specificity Binds to human and mouse ST2.
Biological Activity Activates human and mouse ST2-dependent NF-κB pathway.
MW ~18kDa
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.01EU/μg purified protein (LAL test; Lonza).
Concentration 0.1mg/ml after reconstitution.
Reconstitution Reconstitute with 100μl sterile water.
Formulation Lyophilized. Contains PBS.
Other Product Data UniProt link Q2YEJ5: Interleukin-33 (human)
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Interleukin-33 (IL-33; HF-NEV; IL-1F11), a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released upon cell lysis. IL33 binds to and signals through ST2 (IL1R1) and its stimulation recruits MYD88, IRAK, IRAK4, and TRAF6, followed by phosphorylation of ERK1(MAPK3)/ERK2(MAPK1), p38(MAPK14), and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells, and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases, and sepsis.

Product References
  1. An IL-1 cytokine member, IL-33, induces human basophil activation via its ST2 receptor: M. Suzukawa, et al.; J. Immunol. 181, 5981 (2008)
  2. Leptin enhances survival and induces migration, degranulation, and cytokine synthesis of human basophils: M. Suzukawa, et al.; J. Immunol. 186, 5254 (2011)
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