AdipoGen Life Sciences

anti-Vaspin (human), mAb (VP63)

CHF 310.00

In stock

AG-20A-0045-C05050 µgCHF 310.00

AG-20A-0045-C100100 µgCHF 430.00

Figure 1: Immunohistochemical staining of vaspin with anti-Vaspin (human), mAb (VP63) (AG-20A-0045) in psoriatic skin lesions (A). Negative control for vaspin in psoriatic skin lesions (B). Blue hematoxylin stained cell nuclei.

Figure 2: Immunohistochemical staining of vaspin with anti-Vaspin (human), mAb (VP63) (AG-20A-0045) in healthy skin (A). Arrow - stronger staining in Stratum granulosum. Negative control for vaspin in healthy skin (B). Blue hematoxylin stained cell nuclei.

Figure 3: Western blot analysis using anti-Vaspin (human), mAb (VP63) (Prod. No. AG-20A-0045) at 1:1'000 dilution.
1: Human vaspin (His-tagged).
2: Human vaspin (FLAG^®-tagged).
3: Mouse IL-33 (His-tagged) (negative control).

Specifications / Handling

More Information
Product Details
Synonyms	Visceral Adipose Tissue-derived Serine Protease Inhibitor; Serpin A12; OL-64
Product Type	Monoclonal Antibody
Properties
Clone	VP63
Isotype	Mouse IgG1κ
Immunogen/Antigen	Recombinant human vaspin.
Application	ELISA: (direct or indirect: 1:2'000-1:5'000) Immunohistochemistry: frozen sections (1:100) Western blot: (1:2'000-1:5'000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application.
Crossreactivity	Human
Specificity	Recognizes human vaspin. Detects a band of ~48kDa by Western blot.
Purity Detail	Protein G-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Mouse IgG1 Isotype Control
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Vaspin (Visceral adipose tissue-derived serpin; Serpin A12), a serine protease inhibitor (serpin), is an insulin-sensitizing adipocytokine that has been isolated from both visceral and subcutaneous white adipose tissue. Based on recent findings, vaspin is suggested to regulate immune responses and inflammation and was found to be correlated with various metabolic parameters. Vaspin may represent a novel biomarker for obesity and impaired insulin sensitivity and might serve as a new therapeutic target of metabolic syndrome.

Product References

Central vaspin administration acutely reduces food intake and has sustained blood glucose-lowering effects: N. Klöting, et al.; Diabetologia 54, 1819 (2011)
Untersuchungen zur Rolle von Vaspin in der Psoriasis: K. Rall; Diss. Universität Leipzig (2012) (IHC protocol)

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