AdipoGen Life Sciences

anti-ANGPTL4 (coiled-coil domain) (human), mAb (Kairos4-397G)

CHF 270.00

In stock

AG-20A-0047-C05050 µgCHF 270.00

AG-20A-0047-C100100 µgCHF 350.00

Figure 1: Immunohistochemical staining of ANGPTL4 using anti-ANGPTL4 (coiled-coil domain) (human), mAb (Kairos4-397G) (Prod. No. AG-20A-0047) in human placenta tissue (10µg/ml).
This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

Figure 2: Western blot analysis using anti-ANGPTL4 (CCD) (human), mAb (Kairos4-397G) (Prod. No. AG-20A-0047) at 1:500 dilution.
1: Human ANGPTL4 (CCD) (FLAG^®-tagged).
2: Human ANGPTL4 (FLD) (FLAG^®-tagged).
3: Human ANGPTL4 (FLAG^®-tagged).
4: Human RBP4 (FLAG^®-tagged) (negative control).
5: Human ANGPTL3 (CCD) (FLAG^®-tagged).
6: Human ANGPTL2 (CCD) (FLAG^®-tagged).
7: Human ANGPTL5 (CCD) (FLAG^®-tagged).
8: Human ANGPTL3 (FLAG^®-tagged).

Specifications / Handling

More Information
Product Details
Synonyms	Angiopoietin-like Protein 4; FIAF; Fasting-induced Adipose Factor; HFARP; Hepatic Fibrinogen/Angiopoietin-related Protein
Product Type	Monoclonal Antibody
Properties
Clone	Kairos4-397G
Isotype	Mouse IgG1κ
Immunogen/Antigen	Recombinant human coiled-coil domain of ANGPTL4.
Application	ELISA: (direct or indirect: 1:500-1:5'000) Immunohistochemistry: (10μg/ml) Western blot: (1:500-1:5'000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions should be determined individually for each application.
Crossreactivity	Human
Specificity	Recognizes the coiled-coil domain (CCD) of human ANGPTL4. Detects a band of ~19kDa by Western blot.
Purity Detail	Protein G-affinity purified.
Concentration	See on label.
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Mouse IgG1 Isotype Control
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

ANGPTL4 mainly expressed in endothelial cells (hypoxia-induced). Regulates angiogenesis and modulates tumorgenesis and directly regulates lipid, glucose, and energy metabolism. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. ANGPTL4 is a protein consisting of an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain (FLD). Both domains have distinct biological functions. The coiled-coil domain is responsible for the inhibitory effects on lipoprotein lipase (LPL) converting the active form of LPL into an inactive form, and the FLD domain mediates its antiangiogenic functions. The coiled coil and the FLD domains are separated by a short linker that can be cleaved after secretion. ANGPTL4 appears on the cell surface as the full-length form, where it can be released by heparin treatment. ANGPTL4 protein is then proteolytically cleaved by proprotein convertases (PCs), including furin, PC5/6, paired basic amino acid-cleaving enzyme 4, and PC7.

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