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Figure 1: Immunohistochemical staining of ANGPTL4 using anti-ANGPTL4 (coiled-coil domain) (human), mAb (Kairos4-397G) (Prod. No. AG-20A-0047) in human placenta tissue (10µg/ml). This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Figure 2: Western blot analysis using anti-ANGPTL4 (CCD) (human), mAb (Kairos4-397G) (Prod. No. AG-20A-0047) at 1:500 dilution. 1: Human ANGPTL4 (CCD) (FLAG®-tagged). 2: Human ANGPTL4 (FLD) (FLAG®-tagged). 3: Human ANGPTL4 (FLAG®-tagged). 4: Human RBP4 (FLAG®-tagged) (negative control). 5: Human ANGPTL3 (CCD) (FLAG®-tagged). 6: Human ANGPTL2 (CCD) (FLAG®-tagged). 7: Human ANGPTL5 (CCD) (FLAG®-tagged). 8: Human ANGPTL3 (FLAG®-tagged).
Angiopoietin-like Protein 4; FIAF; Fasting-induced Adipose Factor; HFARP; Hepatic Fibrinogen/Angiopoietin-related Protein
Product Type
Monoclonal Antibody
Properties
Clone
Kairos4-397G
Isotype
Mouse IgG1κ
Immunogen/Antigen
Recombinant human coiled-coil domain of ANGPTL4.
Application
ELISA: (direct or indirect: 1:500-1:5'000) Immunohistochemistry: (10μg/ml) Western blot: (1:500-1:5'000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions should be determined individually for each application.
Crossreactivity
Human
Specificity
Recognizes the coiled-coil domain (CCD) of human ANGPTL4. Detects a band of ~19kDa by Western blot.
Purity Detail
Protein G-affinity purified.
Concentration
See on label.
Formulation
Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
ANGPTL4 mainly expressed in endothelial cells (hypoxia-induced). Regulates angiogenesis and modulates tumorgenesis and directly regulates lipid, glucose, and energy metabolism. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. ANGPTL4 is a protein consisting of an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain (FLD). Both domains have distinct biological functions. The coiled-coil domain is responsible for the inhibitory effects on lipoprotein lipase (LPL) converting the active form of LPL into an inactive form, and the FLD domain mediates its antiangiogenic functions. The coiled coil and the FLD domains are separated by a short linker that can be cleaved after secretion. ANGPTL4 appears on the cell surface as the full-length form, where it can be released by heparin treatment. ANGPTL4 protein is then proteolytically cleaved by proprotein convertases (PCs), including furin, PC5/6, paired basic amino acid-cleaving enzyme 4, and PC7.
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