AdipoGen Life Sciences

anti-FTO (mouse), mAb (FT62-6)

CHF 300.00

In stock

AG-20A-0083-C05050 µgCHF 300.00

AG-20A-0083-C100100 µgCHF 390.00

Figure 1: Immunohistochemical staining of mouse FTO using anti-FTO (mouse), mAb (FT62-6) (Prod. No. AG-20A-0083) in mouse tissue (1:500 dilution, 200X).
A. Immunoperoxidase staining of formalin-Fixed, paraffin-embedded mouse brain.
A-1. Isotype control, mouse brain.
B. Immunoperoxidase staining of formalin-Fixed, paraffin-embedded mouse uterus.
B-1. Isotype control, mouse uterus.

Figure 2: Immunoprecipitation confirmed by recombinant mouse FTO. Recombinant mouse FTO proteins at different concentrations were precipitated using anti-FTO (mouse), mAb (FT62-6) (Prod. No. AG-20A-0083). The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by western blot with rabbit anti-mouse FTO pAb.
1. mFTO (His-tagged) recombinant control 50ng (AG-40A-127).
2. mFTO (His-tagged) 10μg.
3. mFTO (His-tagged) 5μg.
4. mFTO (His-tagged) 2.5μg.
5. mFTO (His-tagged) 1.25μg.
6. mFTO (His-tagged) 0.312μg.
7. mFTO (His-tagged) 0.156μg.

Figure 3: Western blot analysis using anti-FTO (mouse), mAb (FT62-6) (Prod. No. AG-20A-0083) at 1:2'000 dilution.
A)
1: Mouse kideny (200μg).
2: Mouse spleen (200μg).
3: Mouse heart (200μg).
4: Mouse liver (500μg).
5: Mouse stomach (200μg).
6: Mouse brain (300μg).
7: Mouse lung (200μg).
B)
1: Mouse FTO (10ng).
2: 3T3L1 (100μg).

Specifications / Handling

More Information
Product Details
Synonyms	Fat Mass and Obesity-associated Protein; FATSO
Product Type	Monoclonal Antibody
Properties
Clone	FT62-6
Isotype	Mouse IgG1κ
Immunogen/Antigen	Recombinant human FTO.
Application	ELISA: (direct and indirect: 1:2’000-1:5’000) Immunohistochemistry: (paraffin sections (1:100-1:500)) Immunoprecipitation: (5μg/ml per sample in a final reaction volume of 500-1000μl). Western Blot: (1:2’000-1:5’000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
Crossreactivity	Mouse
Specificity	Recognizes mouse FTO. Weakly cross-reacts with human FTO. Does not cross-react with rat FTO.
Purity Detail	Protein G-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Mouse IgG1 Isotype Control
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

FTO (Fat mass-and obesity-associated gene) is the responsible gene for mouse ‘fused toes’ mutation. An association between FTO genotype and type 2 diabetes has been confirmed. The presence of the FTO rs9939609 A-allele was found to be positively correlated with other symptoms of the metabolic syndrome, including higher fasting insulin, glucose, triglycerides, and lower HDL-cholesterol.

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