AdipoGen Life Sciences

anti-FTO (mouse), pAb

CHF 190.00

In stock

AG-25A-0089-C100100 µgCHF 190.00

Figure 1: Immunohistochemical staining (cytoplasmic) of mouse tissue using anti-FTO (mouse), pAb (Prod. No. AG-25A-0089) at 1:500 dilution.
Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse salivary gland in the presence of control peptide (left) or blocking peptide (right) (200x, brown colour).

Figure 2: Western blot analysis of mouse FTO using anti-FTO (mouse), pAb (Prod. No. AG-25A-0089) at 1:4,000 dilution.
1. Recombinant mouse FTO (His-tagged) (100ng).
2. Recombinant human FTO (His-tagged) (100ng).
3. Recombinant hFGF19 (His-tagged) (Negative control) (100ng).
4. Mouse Liver tissue lysate (500μg).

Specifications / Handling

More Information
Product Details
Synonyms	Fat Mass and Obesity-associated Protein; FATSO
Product Type	Polyclonal Antibody
Properties
Source/Host	Rabbit
Immunogen/Antigen	Recombinant mouse FTO.
Application	ELISA: (direct and indirect: 1:2’000-1:5’000) Immunohistochemistry: (paraffin sections 1:500) Western Blot: (1:2’000-1:5’000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application. Note: Tested on recombinant proteins and/or target-protein transfected cell lines in ELISA, Western Blot and/or FACS.
Crossreactivity	Mouse
Specificity	Recognizes mouse FTO. Detects a band of ~58kDa by Western blot. Weakly cross-reacts with human FTO.
Purity Detail	Protein A-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Rabbit IgG
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

FTO (Fat mass-and obesity-associated gene) is the responsible gene for mouse ‘fused toes’ mutation. An association between FTO genotype and type 2 diabetes has been confirmed. The presence of the FTO rs9939609 A-allele was found to be positively correlated with other symptoms of the metabolic syndrome, including higher fasting insulin, glucose, triglycerides, and lower HDL-cholesterol.

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