AdipoGen Life Sciences

anti-FTO (mouse), pAb

CHF 190.00
In stock
AG-25A-0089-C100100 µgCHF 190.00
More Information
Product Details
Synonyms Fat Mass and Obesity-associated Protein; FATSO
Product Type Polyclonal Antibody
Properties
Source/Host Rabbit
Immunogen/Antigen Recombinant mouse FTO.
Application

ELISA: (direct and indirect: 1:2’000-1:5’000)
Immunohistochemistry: (paraffin sections 1:500)
Western Blot: (1:2’000-1:5’000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.

Note: Tested on recombinant proteins and/or target-protein transfected cell lines in ELISA, Western Blot and/or FACS.

Crossreactivity Mouse
Specificity

Recognizes mouse FTO. Detects a band of ~58kDa by Western blot. Weakly cross-reacts with human FTO.

Purity Detail Protein A-affinity purified.
Concentration 1mg/ml
Formulation Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control

Rabbit IgG

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

FTO (Fat mass-and obesity-associated gene) is the responsible gene for mouse ‘fused toes’ mutation. An association between FTO genotype and type 2 diabetes has been confirmed. The presence of the FTO rs9939609 A-allele was found to be positively correlated with other symptoms of the metabolic syndrome, including higher fasting insulin, glucose, triglycerides, and lower HDL-cholesterol.

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