anti-ZBP1, mAb (Zippy-1)

CHF 490.00
In stock
AG-20B-0010-C100100 µgCHF 490.00
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Product Details
Synonyms Z-DNA-binding Protein 1; Tumor Stroma and Activated Macrophage Protein DLM-1; DNA-dependent Activator of IFN-regulatory Factors; DAI
Product Type Monoclonal Antibody
Properties
Clone Zippy-1
Isotype Mouse IgG2a
Source/Host Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen Recombinant mouse ZBP1 (aa 1-411).
Application Immunocytochemistry: (1:500) (see reference 1)
Immunoprecipitation: (1:200)
Western Blot: (1:1’000)
Crossreactivity Human
Mouse
Specificity Recognizes human and mouse ZBP1.
Purity ≥95% (SDS-PAGE)
Purity Detail Protein G-affinity purified.
Concentration 1mg/ml
Formulation Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

ZBP1 is an interferon inducible protein that binds to cytoplasmic DNA and plays a role in host defense against tumors and pathogens. ZBP1 is highly expressed in lymphatic tissues including lymph node, leukocytes, tonsil, bone marrow and spleen. ZBP1 is an innate immune sensor of Influenza A virus (IAV). ZBP1 regulates NLRP3 (nucleotide and oligomerization domain, leucine-rich repeat-containing protein family, pyrin domain containing 3) inflammasome activation as well as induction of different types of cell death (apoptosis, necroptosis and pyroptosis) during IAV infection.

Product References
  1. ZBP1/DAI is an innate sensor of influenza virus triggering the NLRP3 inflammasome and programmed cell death pathways: T. Kuriakose, et al.; Sci. Immunol. 1, aag2045 (2016)
  2. DAI senses Influenza A virus genomic RNA and activates RIPK3-dependent cell death: R.J. Thapa, et al.; Cell Host & Microbe 20, 674 (2016)
  3. RIPK1 counteracts ZBP1-mediated necroptosis to inhibit inflammation: J. Lin, et al.; Nature 540, 124 (2016)
  4. RIPK1 inhibits ZBP1-driven necroptosis during development: K. Newton, et al.; Nature 540, 129 (2016)
  5. IRF1 Is a Transcriptional Regulator of ZBP1 Promoting NLRP3 Inflammasome Activation and Cell Death during Influenza Virus Infection: T. Kuriakose, et al.; J. Immunol. ahead of print (2018)
  6. Constitutive interferon signaling maintains critical threshold of MLKL expression to license necroptosis: J. Sarhan, et al.; Cell Death Diff. ahead of print (2018)
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