AdipoGen Life Sciences

anti-BAFF-R (mouse), mAb (9B9) (ATTO 647N)

CHF 440.00
In stock
AG-20B-0034TS-T100100 testsCHF 440.00
More Information
Product Details
Synonyms BAFF Receptor; BR3; BlySR3; CD268; TNFRSF13C
Product Type Monoclonal Antibody
Properties
Clone 9B9
Isotype Rat IgG2b
Source/Host Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen Y3 cells transfected with mouse BAFF-R.
Label/Conjugates ATTO 647N
Application Flow Cytometry
Functional Application: Depletion of B cells in vivo.
Optimal conditions must be determined individually for each application.

Definition for 1 test: 1μl of labeled antibody in 200μl of Flow Cytometry buffer (see online protocol).
Crossreactivity Mouse
Specificity Recognizes mouse BAFF-R.
Purity ≥95% (SDS-PAGE)
Purity Detail Protein G-affinity purified.
Concentration 1mg/ml
Formulation Liquid. In PBS containing 0.02% sodium azide.
Other Product Data New ATTO-fluorescent antibodies show increased photostability, outstanding brightness and intense signals. ATTO dyes are thermally stable, resistant to environmental changes and show no significant isomerization.
ATTO 647N shows red fluorescence (λabs (max): 645nm, λem (max): 669nm, εmax: 120’000).
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage +4°C
Handling Advice Do not freeze.
Protect from light.
Use/Stability Keep conjugated formats at +4°C.
Stable for at least 1 year after receipt when stored at +4°C.
Documents
Protocols Download PDF
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

Three receptors for BAFF were recently identified and designated TACI, BCMA and BAFF-R. BAFF-R signaling is essential for B cell development, survival and humoral immune responses.

Product References
  1. Crucial role for BAFF-BAFF-R signaling in the survival and maintenance of mature B cells: M. Rauch, et al.; PLoS ONE 4, e5456 (2009)
  2. BAFF-R expression correlates with positive selection of immature B cells: R. Tussiwand, et al.; Eur. J. Immunol. 42, 206 (2012)
  3. PD-L1hi B cells are critical regulators of humoral immunity: A.R. Khan, et al.; Nat. Commun. 6, 5997 (2015)
  4. The unknown aspect of BAFF: Inducing IL-35 production by a CD5+CD1dhiFcγRIIbhi regulatory B-Cell subset in lupus: Y. Zhang, et al.; J. Invest. Dermatol. 137, 2532 (2017)
  5. B-1a lymphocytes promote oligodendrogenesis during brain development: S. Tanabe & T. Yamashita; Nature Neurosci. 21, 506 (2018)
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