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AdipoGen Life Sciences
anti-NAIP1/2/5 (mouse), mAb (Naipa-1)
480
CHF
CHF 480.00
In stock
AG-20B-0045-C100100 µgCHF 480.00
Figure 1: Overexpressed mouse NAIP1, 2 and 5 are detected by immunoblotting using anti-NAIP1/2/5 (mouse), mAb (Naipa-1) (Prod. No. AG-20B-0045). Method: Cell extracts from HEK293T either transfected with a plasmid coding for mouse Flag-NAIP1, 2 or 5 (lanes 2, 3, 4) or a negative control (GFP) (lane 1), were separated by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with anti-NAIP1/2/5 (mouse), mAb (Naipa-1 (1:1000) or anti-Flag (M2) (1:1000) in incubation buffer (0.4% skimmed milk / PBS / Tween 20) followed by anti-mouse HRP (1:10‘000) for 1h at RT. Exposure 10 seconds.
| Product Details | |
|---|---|
| Synonyms | Neuronal Apoptosis Inhibitory Protein 1, 2, 5; Baculoviral IAP Repeat-containing Protein 1a, 1b, 1e; Birc 1a, 1b, 1e |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | Naipa-1 |
| Isotype | Mouse IgG2bκ |
| Source/Host | Purified from concentrated hybridoma tissue culture supernatant. |
| Immunogen/Antigen | Recombinant mouse NAIP5 (BIR2 domain/aa 159-227). |
| Application |
Western Blot: (1μg/ml) |
| Crossreactivity | Mouse |
| Specificity |
Recognizes mouse NAIP1, 2 and 5. Not tested on mouse NAIP6 and mouse NAIP7 but should also work due to high homology in the BIR2 domain. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein G-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. |
| Isotype Negative Control | |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
The inflammasome is a multiprotein complex that mediates the activation of caspase-1, which promotes, amongst others, the secretion of the proinflammatory cytokines interleukin (IL)-1β and IL-18. Members of the Nod-like receptor (NLR) family are critical components of the inflammasome that link danger-signals to caspase-1 activation. The NLR member IPAF/NLRC4 senses bacterial flagellin and a conserved type III secretion system (TTSS) rod component. A member of the NLR family, NAIP5 directly and specifically interacts with flagellin, triggers a physical NAIP5-NLRC4 association leading to NLRC4 activation. The related NAIP2 functions analogously to NAIP5, serving as a specific inflammasome receptor for TTSS rod proteins such as Salmonella PrgJ and Burkholderia BsaK.
Product References
- CD137 ligand-CD137 interaction is required for inflammasome-associated brain injury following ischemic stroke: D.Y. Fann, et al.; NeuroMol. Med. 22, 474 (2020)
- Kinetics of free and ligand-bound atacicept in human serum: M. Eslami, et al.; Front. Immunol. 13, 1035556 (2022)
- Inflammasome Activation Mediates Apoptotic and Pyroptotic Death in Astrocytes Under Ischemic Conditions: LJ. Wong, et al.; Neuromolecular Med. ahead of print (2023)
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