anti-IL-1α (p18) (mouse), mAb (Teo-1)
Method: IL-1α was analyzed by Western blot in cell extracts of bone marrow-derived dendritic cells (BMDCs) treated by LPS and the several inflammasome activators as indicated in the figure. Cell extracts were separated by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with anti-IL-1α (p18) (mouse), mAb (Teo-1) (1µg /ml). After addition of an anti-mouse secondary antibody coupled to HRP, proteins were visualized by a chemiluminescence detection system.
|Product Type||Monoclonal Antibody|
|Source/Host||Purified from concentrated hybridoma tissue culture supernatant.|
|Immunogen/Antigen||Recombinant mouse mature IL-1α.|
Western Blot: (1μg/ml)
|Specificity||Recognizes mouse IL-1α p18 cleaved and full-length fragments.|
|Purity Detail||Protein G-affinity purified.|
|Formulation||Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
|Use/Stability||Stable for at least 1 year after receipt when stored at -20°C.|
|Product Specification Sheet|
The most prominent members of the interleukin-1 (IL-1) superfamily are IL-1α and IL-1β. They lack a signal peptide and are secreted by an unconventional, endoplasmic reticulum-Golgi-independent mechanism. IL-1α was reported to be more widely and constitutively expressed and has intracellular functions, but also acts locally in a membrane-bound form by activating IL-1R1. Additionally, passive release of IL-1α upon cell death can trigger a sterile inflammatory response to dying cells. The cleavage of IL-1α is not mediated by caspase-1 and is not required for binding to IL-1R1. Recently it has been observed that all activators of the inflammasome NLRP3/NALP3 induce the simultaneous secretion of IL-1α and IL-1β. Although most activators fully rely on the inflammasome for IL-1α secretion, some induce the processing and secretion of IL-1α in an inflammasome-independent manner.