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Chimerigen
IL-1α (human) (rec.) (His)
Product Details | |
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Synonyms | Interleukin-1α; IL-1F1; IL-1A |
Product Type | Protein |
Properties | |
Source/Host | E. coli |
Sequence |
Human IL-1α (aa 113-271) is fused at the C-terminus to a His-tag. |
Crossreactivity | Human |
Purity | ≥95% (SDS-PAGE) |
Endotoxin Content | <1EU/μg protein (LAL test; Lonza). |
Reconstitution | Reconstituted in sterile H2O not less than 100μg/ml, which can then be further diluted in other aqueous solutions. |
Formulation | Lyophilized from a concentrated sterile solution containing 50mM Tris-HCl buffer (pH 8.0) and 100mM NaCl. |
Other Product Data |
NCBI reference NP_000566.3: IL-1α (human) |
Declaration | Manufactured by Chimerigen. |
Shipping and Handling | |
Shipping | BLUE ICE |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Handling Advice |
Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. |
Use/Stability |
Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
Documents | |
MSDS | Inquire |
Product Specification Sheet | |
Datasheet | Download PDF |
The most prominent members of the interleukin-1 (IL-1) superfamily are IL-1α and IL-1β. They lack a signal peptide and are secreted by an unconventional, endoplasmic reticulum-Golgi-independent mechanism. IL-1α was reported to be more widely and constitutively expressed and has intracellular functions, but also acts locally in a membrane-bound form by activating IL-1R1. Additionally, passive release of IL-1α upon cell death can trigger a sterile inflammatory response to dying cells. The cleavage of IL-1α is not mediated by caspase-1 and is not required for binding to IL-1R1. Recently it has been observed that all activators of the inflammasome NLRP3/NALP3 induce the simultaneous secretion of IL-1α and IL-1β. Although most activators fully rely on the inflammasome for IL-1α secretion, some induce the processing and secretion of IL-1α in an inflammasome-independent manner.